中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2011年
2期
96-99
,共4页
韦登明%贾学敏%尹向旭%蒋雯雯
韋登明%賈學敏%尹嚮旭%蔣雯雯
위등명%가학민%윤향욱%장문문
血管性痴呆%电针%学习记忆能力%长时程增强效应%突触素
血管性癡呆%電針%學習記憶能力%長時程增彊效應%突觸素
혈관성치태%전침%학습기억능력%장시정증강효응%돌촉소
Vascular dementia%Electroacupuncture%Learning%Memory ability%Long-term potentiation%Synaptophysin
目的 观察电针对血管性痴呆(VD)大鼠学习记忆功能的影响,探讨电针治疗VD的作用机制.方法 将40只Sprague-Dawley大鼠分为假手术组、模型组、电针组和西药组,除假手术组行假手术外,其余各组建立VD大鼠模型.电针组术后给予电针百会、大椎穴治疗,药物组给予尼莫地平灌胃,均治疗10 d.采用Morris水迷宫试验检测大鼠学习记忆能力,微电极记录海马齿状回长时程增强效应,免疫组织化学染色技术观察脑组织海马突触素(Syp)染色情况.结果 与模型组比较,电针组和西药组治疗后水迷宫逃避潜伏期明显缩短(P<0.01),相同时间内在原平台象限跨越相应平台次数明显增多(P<0.01),高频刺激后相对群峰电位潜伏期缩短、相对兴奋性突触后电位斜率和相对群峰电位幅值增大(P<0.05),Syp免疫阳性细胞积分光密度值显著增大(P<0.01).结论 电针大鼠百会、大椎穴能改善大鼠学习记忆能力,其机制可能是通过促进海马Syp的表达从而增强海马突触可塑性以及加快海马神经元突触传递过程来实现.
目的 觀察電針對血管性癡呆(VD)大鼠學習記憶功能的影響,探討電針治療VD的作用機製.方法 將40隻Sprague-Dawley大鼠分為假手術組、模型組、電針組和西藥組,除假手術組行假手術外,其餘各組建立VD大鼠模型.電針組術後給予電針百會、大椎穴治療,藥物組給予尼莫地平灌胃,均治療10 d.採用Morris水迷宮試驗檢測大鼠學習記憶能力,微電極記錄海馬齒狀迴長時程增彊效應,免疫組織化學染色技術觀察腦組織海馬突觸素(Syp)染色情況.結果 與模型組比較,電針組和西藥組治療後水迷宮逃避潛伏期明顯縮短(P<0.01),相同時間內在原平檯象限跨越相應平檯次數明顯增多(P<0.01),高頻刺激後相對群峰電位潛伏期縮短、相對興奮性突觸後電位斜率和相對群峰電位幅值增大(P<0.05),Syp免疫暘性細胞積分光密度值顯著增大(P<0.01).結論 電針大鼠百會、大椎穴能改善大鼠學習記憶能力,其機製可能是通過促進海馬Syp的錶達從而增彊海馬突觸可塑性以及加快海馬神經元突觸傳遞過程來實現.
목적 관찰전침대혈관성치태(VD)대서학습기억공능적영향,탐토전침치료VD적작용궤제.방법 장40지Sprague-Dawley대서분위가수술조、모형조、전침조화서약조,제가수술조행가수술외,기여각조건립VD대서모형.전침조술후급여전침백회、대추혈치료,약물조급여니막지평관위,균치료10 d.채용Morris수미궁시험검측대서학습기억능력,미전겁기록해마치상회장시정증강효응,면역조직화학염색기술관찰뇌조직해마돌촉소(Syp)염색정황.결과 여모형조비교,전침조화서약조치료후수미궁도피잠복기명현축단(P<0.01),상동시간내재원평태상한과월상응평태차수명현증다(P<0.01),고빈자격후상대군봉전위잠복기축단、상대흥강성돌촉후전위사솔화상대군봉전위폭치증대(P<0.05),Syp면역양성세포적분광밀도치현저증대(P<0.01).결론 전침대서백회、대추혈능개선대서학습기억능력,기궤제가능시통과촉진해마Syp적표체종이증강해마돌촉가소성이급가쾌해마신경원돌촉전체과정래실현.
Objective To investigate the effects of electroacupuncture (EA) on spatial learning and memory ability, long-term potentiation (LTP) and synaptophysin (Syp) levels in the dentate gyrus of the hippocampus.Methods Forty Sprague-Dawley rats were randomly divided into a sham-operated control group, a model group, an EA group and a medication group. A rat model of vascular dementia (VD) was established by repeated ischemia and reperfusion after pretreatment with sodium nitroprusside. EA was applied to the rat homologues of the human "Baihui" (GV20) and "Dazhui" (DU14) acupoints of the rats in the EA group for 20 min, once daily for 10 d.Morris water maze tests were conducted for evaluating the rats,learning and memory ability. LTP in the hippocampal dentate gyrus was recorded as an electrophysiological index for evaluating learning and memory. Antigenic Syp produced in hippocampal tissue was examined with immunohistochemical assays. Results In comparison the with model group, escape latency (EL) shortened and target-platform crossing times increased significantly in the EA and medication groups. After high frequency stimulation the population spike (PS) latency shortened in the EA group compared with the model group, and the excitatory postsynaptic potential (EPSP) slope and PS amplitude increased.All of these differences were statistically significant. Syp positive cells also increased significantly in the EA-treated rats. Conclusions EA can increase Syp expression and improve the learning and memory ability of rats with this model of VD. EA could facilitate the induction and maintenance of LTP in the dentate gyrus of the hippocampus through influencing synaptic transmission.