中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2012年
3期
199-202
,共4页
伍丽君%赵天兰%余道江%张为宝%刘强
伍麗君%趙天蘭%餘道江%張為寶%劉彊
오려군%조천란%여도강%장위보%류강
外科皮瓣%基质细胞衍生因子-1%血管新生
外科皮瓣%基質細胞衍生因子-1%血管新生
외과피판%기질세포연생인자-1%혈관신생
Surgical flap%Stromal cell derived factor 1%Neovascularization
目的 检测基质细胞衍生因子-1( stromal cell derived factor1,SDF-1)在缺氧缺血型狭长窄蒂皮瓣组织不同时间、不同面积的表达,研究SDF-1/CXCR4轴在狭长窄蒂皮瓣组织血管新生中的作用.方法 在5头家猪双侧背部均制作蒂部长宽比例为4:2,携带面积分别为2 cm ×2 cm(A组皮瓣)、3 cm × 3 cm(B组皮瓣)、4 cm×4 cm(C组皮瓣)、5 cm × 5 cm(D组皮瓣)和6 cm ×6 cm(E组皮瓣)的狭长窄蒂皮瓣模型.对5组皮瓣进行大体观察、成活面积计算、皮瓣组织HE染色,用ELISA法检测皮瓣组织远端在术中及术后3、5、7、14 d时SDF-1表达变化情况.结果 ①同一组狭长窄蒂皮瓣,随着时间推移,SDF-1的表达也随之增加,在第5天达到最高值,随后逐渐下降,趋于基础水平.②不同组狭长窄蒂皮瓣,随着皮瓣面积的增大,SDF-1的表达也随之增加,当皮瓣面积达5cm×5 cm时,SDF-1的表达量不再增加,皮瓣远端部分坏死.结论 SDF-1的表达量可能与狭长窄蒂皮瓣成活过程中的供血量相关.
目的 檢測基質細胞衍生因子-1( stromal cell derived factor1,SDF-1)在缺氧缺血型狹長窄蒂皮瓣組織不同時間、不同麵積的錶達,研究SDF-1/CXCR4軸在狹長窄蒂皮瓣組織血管新生中的作用.方法 在5頭傢豬雙側揹部均製作蒂部長寬比例為4:2,攜帶麵積分彆為2 cm ×2 cm(A組皮瓣)、3 cm × 3 cm(B組皮瓣)、4 cm×4 cm(C組皮瓣)、5 cm × 5 cm(D組皮瓣)和6 cm ×6 cm(E組皮瓣)的狹長窄蒂皮瓣模型.對5組皮瓣進行大體觀察、成活麵積計算、皮瓣組織HE染色,用ELISA法檢測皮瓣組織遠耑在術中及術後3、5、7、14 d時SDF-1錶達變化情況.結果 ①同一組狹長窄蒂皮瓣,隨著時間推移,SDF-1的錶達也隨之增加,在第5天達到最高值,隨後逐漸下降,趨于基礎水平.②不同組狹長窄蒂皮瓣,隨著皮瓣麵積的增大,SDF-1的錶達也隨之增加,噹皮瓣麵積達5cm×5 cm時,SDF-1的錶達量不再增加,皮瓣遠耑部分壞死.結論 SDF-1的錶達量可能與狹長窄蒂皮瓣成活過程中的供血量相關.
목적 검측기질세포연생인자-1( stromal cell derived factor1,SDF-1)재결양결혈형협장착체피판조직불동시간、불동면적적표체,연구SDF-1/CXCR4축재협장착체피판조직혈관신생중적작용.방법 재5두가저쌍측배부균제작체부장관비례위4:2,휴대면적분별위2 cm ×2 cm(A조피판)、3 cm × 3 cm(B조피판)、4 cm×4 cm(C조피판)、5 cm × 5 cm(D조피판)화6 cm ×6 cm(E조피판)적협장착체피판모형.대5조피판진행대체관찰、성활면적계산、피판조직HE염색,용ELISA법검측피판조직원단재술중급술후3、5、7、14 d시SDF-1표체변화정황.결과 ①동일조협장착체피판,수착시간추이,SDF-1적표체야수지증가,재제5천체도최고치,수후축점하강,추우기출수평.②불동조협장착체피판,수착피판면적적증대,SDF-1적표체야수지증가,당피판면적체5cm×5 cm시,SDF-1적표체량불재증가,피판원단부분배사.결론 SDF-1적표체량가능여협장착체피판성활과정중적공혈량상관.
Objective To detect the stromal cell derived factor 1 (SDF-1) expression during the survival process of the narrow pedicle flap with hypoxia and ischemia and to investigate the role of SDF-1/CXCR4 axis in flap neovascularization.Methods The narrow pedicle flaps were formed on the bilateral back of 5 pigs.The pedicle ratio of length to width was 4:2.The flap size was 2 cm ×2 cm(group A),3 cm ×3 cm(group B),4 cm ×4 cm(group C),5 cm ×5 cm(group D),6 cm ×6 cm(group E).The flaps survival rate was observed and HE staining was performed.The SDF-1 expression at the distal end of flaps was detected by ELISA during the operation and 3,5,7,14 days after operation.Results ① SDF-1 expression at the same group increased after operation until it reached the peak value at 5 days after operation; then it decreased to basic value.② SDF-1 expression in different groups was higher in bigger flaps until the flaps size reached 5 cm× 5 cm.Then partial necrosis happened at the distal end of flaps.Conclusions The SDF-1 expression may be related to the blood supply during the survival process of the narrow pedicle flap with hypoxia and ischemia.
