CAJ | 학술논문

植物嫁接可以导致接穗的后代中有可遗传性变异的发生.我们将绿豆(Vigna radiata (L.) Wilczek)的幼苗嫁接在红薯(Ipomoea batatas (L.) Lam.)的茎上, 维护其生长至结实.将收获的绿豆种子连续几代播种于普通环境时, 其后代中出现了明显的遗传变异.这些变异在未经嫁接的绿豆接穗品系中并不出现.为了研究这种嫁接诱导变异现象可能的机理, 我们对变异品系的细胞质和细胞核DNA进行了分析.结果显示,在原绿豆和变异品系之间未发现细胞质DNA的RFLP(限制性酶切片段多态性)差异.而细胞核DNA却发生了高频率的序列重组.同时,本研究没有发现砧木与接穗间基因转移的迹象.根据以上事实,我们推测远缘嫁接变异很有可能是嫁接生长逆境诱导的抗逆变异.
식물가접가이도치접수적후대중유가유전성변이적발생.아문장록두(Vigna radiata (L.) Wilczek)적유묘가접재홍서(Ipomoea batatas (L.) Lam.)적경상, 유호기생장지결실.장수획적록두충자련속궤대파충우보통배경시, 기후대중출현료명현적유전변이.저사변이재미경가접적록두접수품계중병불출현.위료연구저충가접유도변이현상가능적궤리, 아문대변이품계적세포질화세포핵DNA진행료분석.결과현시,재원록두화변이품계지간미발현세포질DNA적RFLP(한제성매절편단다태성)차이.이세포핵DNA각발생료고빈솔적서렬중조.동시,본연구몰유발현침목여접수간기인전이적적상.근거이상사실,아문추측원연가접변이흔유가능시가접생장역경유도적항역변이.
Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean (Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato (Ipomoea batatas (L.) Lam.). The growth of the scion was maintained until the scion produced selfed seeds. We sowed the seeds for several generations under normal conditions. Distinct genetic variations appeared in the progenies. Similar variations did not appear in the generations of the scion sowed normally without graft. The variations seemed to be induced by the graft and they inherited steadily. For understanding the possible mechanism of the phenomenon (graft-induced inheritable variation), we analyzed the cytoplasmic and genomic DNA of the variations. The results showed that there was no restriction fragment length polymorphism (RFLP) in the cytoplasmic DNA between the original scion and the variation. However, significant difference between the scion and variation was recognized by random amplified polymorphic DNA (RAPD) analysis. In addition, there was no evidence that indicated the gene transformation from stock to scion. Our results suggest that the non-specific grafting has a pragmatic potential for plant breeding and crop improvement and, the genetic variation seems not to be caused simply by DNA transformation but most likely the stress induced mutation.