中国人民解放军军医大学学报
中國人民解放軍軍醫大學學報
중국인민해방군군의대학학보
JOURNAL OF MEDICAL COLLEGES OF PLA
2004年
4期
211-213
,共3页
connective tissue growth factor%antisense oligonucleotides%keloid%fibroblast%proliferation%collagensynthesis
Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblasts (HKFs) culture media. The intracellular distribution of CTGF ASODN was observed by fluorescence microscopy in the fixed HKFs. The proliferation of HKFs was measured by MTT test. The collagen synthesis of HKFs was measured by 3H-proline incorporation method. Results: Compared with control group, the CTGF ASODN can inhibit the proliferation and collagen synthesis of the HKFs (P ＜ 0.01 ). Conclusion: CTGF ASODN has anti-fibrotic effects on keloid in vitro, and CTGF play an important role in promoting the fibrosis of keloid.
