中南工业大学学报(英文版)
中南工業大學學報(英文版)
중남공업대학학보(영문판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY(ENGLISH EDITION)
2004年
3期
295-299
,共5页
黄兰芳%吴名剑%孙贤军%郭方遒%梁逸曾%李晓如
黃蘭芳%吳名劍%孫賢軍%郭方遒%樑逸曾%李曉如
황란방%오명검%손현군%곽방주%량일증%리효여
high performance liquid chromatography%electrospray ionization interface%mass spectrometry%cordyceps sinensis%nucleoside
A simple, sensitive and reproducible high performance liquid chromatography-mass spectrometry coupled with electrospray ionization method for simultaneous separation and determination of adenine, adenosine and uridine was developed. The analytical column is a 2.0 mm× 150 mm Shimadzu VP-ODS column and volume fraction of the mobile phase is 86.5 %water, 12.0%methanol and 1.5%formic acid. 2-chloroadenosine was used as internal standard. Selective ion monitoring mode and selective ion monitoring ions at ratio of mass to electric charge of 136 for adenine, 268 for adenosine and 267 for uridine were chosen for quantitative analysis of the three active components. The results show that the regression equations and linear range are Y=0. 062X+0. 005 and 2.0 - 140.0μg · mL 1for adenine, Y=0. 049X+0. 004 and 4. 0 - 115.0 μg · mL-1 for uridine, Y=0. 154X+0. 014 and 1.0 - 125.0 μg · mL 1 for adenosine. The limits of detection are 0.6 μg · mL 1 for adenine, 1.0μg · mL-1 for uri dine and 0.2 μg · mL 1 for adenosine.The recoveries of the three constituents are from 96.6% to 103.2%.