CAJ | 학술논문

采用分步醇沉法对铁皮石斛总多糖(DSP)进行分离,得到乙醇终浓度为40%(DSP3)、70%(DSP2)和90%(DSP)的多糖.进一步对4种铁皮石斛多糖的抗氧化活性进行测定,结果显示:DSP_1对DPPH·的清除作用、总抗氧化能力、抑制H_2O_2诱导红细胞氧化溶血和抑制Fe~(2+)-VC诱导的小鼠肝匀浆脂质过氧化作用效果最佳,DSP和DSP_3次之,DSP_2相对较弱;对·OH的清除率大小依次为DSP>DSP_3>DSP_1>DSP_2;且不同相对分子质量的铁皮石斛多糖均可显著抑制羟基自由基介导的DNA氧化断裂.结果提示:不同相对分子质量铁皮石斛多糖均有抗氧化作用,且抗氧化能力与其相对分子质量大小有关.
채용분보순침법대철피석곡총다당(DSP)진행분리,득도을순종농도위40%(DSP3)、70%(DSP2)화90%(DSP)적다당.진일보대4충철피석곡다당적항양화활성진행측정,결과현시:DSP_1대DPPH·적청제작용、총항양화능력、억제H_2O_2유도홍세포양화용혈화억제Fe~(2+)-VC유도적소서간균장지질과양화작용효과최가,DSP화DSP_3차지,DSP_2상대교약;대·OH적청제솔대소의차위DSP>DSP_3>DSP_1>DSP_2;차불동상대분자질량적철피석곡다당균가현저억제간기자유기개도적DNA양화단렬.결과제시:불동상대분자질량철피석곡다당균유항양화작용,차항양화능력여기상대분자질량대소유관.
Crude polysaccharide from Dendrobium candidum (DSP) was successively fractionated with different concentration ethanol and three polysaccharides named DSP_1, DSP_2 and DSP_3 were obtained at the concentrations of 90%, 70% and 40%, respectively. Subsequently, DSP and its three fractions were tested for antioxidant activity in vitro. Results showed that DSP_1 had the strongest DPPH radical scavenging activity, total antioxidant activity, inhibition against Fe~(2+)-VC-induced lipid peroxidation and inhibition against erythrocyte hemolysis induced by H_2O_2, DSP and DSP_3 took the second place and DSP_2. had the weakest biological activities. The hydroxyl radical scavenging capacity of these test samples followed the order of DSP > DSP_3 > DSP_1> DSP_2. In addition, all fractions of Dendrobium candidum polysaccharides showed potential for protecting against DNA damage mediated by hydroxyl radicals. These results suggested that polysaccharides from Dendrobium candidum had antioxidant potential, which might be closely associated with their molecule weight.