CAJ | 학술논문

建立了基于氢化物发生火焰原子吸收光谱法测定血清中硒的方法.血样在180±10℃的条件下用硝酸-高氯酸进行快速消解,然后用浓盐酸将6价硒还原为4价硒,再用硼氢化钠将4价硒还原为硒化氢,血清经消解和稀释后取样4.0μg进行测定.该方法具有灵敏、准确、快速和简便等诸多优点,其中的特征浓度、检出限、变异系数、回收率和线形范围分别为2.93μg/L、1.55μg/L、3.3%-5.4%、99.2%-103.4%和1.65-320.0μg/L.对国产氢化物发生器的普及有重大的现实意义.
건립료기우경화물발생화염원자흡수광보법측정혈청중서적방법.혈양재180±10℃적조건하용초산-고록산진행쾌속소해,연후용농염산장6개서환원위4개서,재용붕경화납장4개서환원위서화경,혈청경소해화희석후취양4.0μg진행측정.해방법구유령민、준학、쾌속화간편등제다우점,기중적특정농도、검출한、변이계수、회수솔화선형범위분별위2.93μg/L、1.55μg/L、3.3%-5.4%、99.2%-103.4%화1.65-320.0μg/L.대국산경화물발생기적보급유중대적현실의의.
The selenium in blood serum blood samples were dispelled under 180±10℃ by nitric acid-perchloric acid,then were reduced to quadrivalence selenium by hydrochloric acid,and further to hydrogen selenide by hydroboration sodium,and determined by hydride generation atomic absorptionspectrometry.The method i8 sensitive,accurate,fast and simple.The characteristic concentration,detection limit,the coefficient of variation,the recovery and linear scope were 2.93μg/L,1.55μg/L,3.3%-5.4%,99.2%-103.4%and 1.65-320.0μg/L,respectively.The method has great practical significance for the domestically produced hydride generator's popularization.