CAJ | 학술논문

目的采用血清药理学方法,研究平肺口服液对人胚肺成纤维细胞增殖及诱导细胞凋亡的作用.方法平肺口服液10g/kg和20 g/kg分别灌服大鼠,第1～7天每日一次,第8～10天每日两次,于第11天早上末次灌服后2 h腹主动脉无菌采血制备含药血清.采用噻唑蓝(MTT)比色法和流式细胞术分别检测平肺口服液含药血清对人胚肺成纤维细胞(CCC-HPF-1)增殖、细胞凋亡和细胞周期的影响.结果平肺口服液20 g/kg灌服所得大鼠含药血清作用96 h和120 h分别能抑制CCC-HPF-1细胞增殖(P<0.01);流式细胞术检测表明该含药血清作用96 h能诱导CCC-HPF-1细胞发生凋亡(P<0.05)和G1/G0期阻滞(P<0.05).结论平肺口服液能抑制肺成纤维细胞增殖,诱导细胞凋亡以及使细胞周期阻滞于G1/G0期,这可能有助于其预防放射性肺损伤.
목적 채용혈청약이학방법,연구평폐구복액대인배폐성섬유세포증식급유도세포조망적작용.방법 평폐구복액10g/kg화20 g/kg분별관복대서,제1～7천매일일차,제8～10천매일량차,우제11천조상말차관복후2 h복주동맥무균채혈제비함약혈청.채용새서람(MTT)비색법화류식세포술분별검측평폐구복액함약혈청대인배폐성섬유세포(CCC-HPF-1)증식、세포조망화세포주기적영향.결과 평폐구복액20 g/kg관복소득대서함약혈청작용96 h화120 h분별능억제CCC-HPF-1세포증식(P<0.01);류식세포술검측표명해함약혈청작용96 h능유도CCC-HPF-1세포발생조망(P<0.05)화G1/G0기조체(P<0.05).결론 평폐구복액능억제폐성섬유세포증식,유도세포조망이급사세포주기조체우G1/G0기,저가능유조우기예방방사성폐손상.
Objective To determine the effects of Pingfei Oral Liquid (PFOL),which is a Traditional Chinese medicine (TCM) prescription, on the growth, apoptosis and cell cycle of human lung fibroblasts. Methods Serum containing PFOL was prepared by serologic pharmacology method . PFOL was administered orally to SD rats at doses of 10 g/kg and 20 g/kg respectively, once a day from the 1st to 7 th day, and twice a day from the 8th to 10th day. Blood was obtained from abdominal aorta aseptically at the 11th day 2 hours after the last morning administration and then the serum was separated. The proliferation of human lung fibroblasts (CCC-HPF-1 )after exposure to the serum was measured by MTT assay. The flow cytometry was used to detect the apoptosis and cell cycle of CCC-HPF-1. Results The growth of CCC-HPF-1 could be suppressed at the 96 h and 120 h after exposure to the serum from rats treated with 20 g/kg PFOL (P < 0.01). The cells were also induced apoptosis (P < 0.05 )and arrested at G1/G0 phase at 96 h after exposure to the serum (P < 0.05). Conclusion PFOL could inhibit proliferation and induce apoptosis of lung fibroblasts, which may contribute to its protective effect on radiation-induced lung injury.