CAJ | 학술논문

目的观察大鼠实验性慢性胰腺炎(CP)进展过程中胰腺组织代谢产物变化特征,探讨其在CP分级中的价值.方法 Wistar大鼠36只,经尾静脉注入二丁基二氯化物(dibutyltin dichloride,DBTC)溶液8 mg/kg体重制备CP模型.注射后即刻(对照组)及7、14、21、28、35 d分批取大鼠胰腺组织,小部分行病理检查,大部分液氮冷冻后置-80℃保存.应用高分辨魔角旋转核磁共振波谱(HRMAS NMR)法检测胰腺组织的代谢产物.以非正常结构、管状复合物、腺体萎缩、纤维化、水肿及炎细胞浸润程度等作为基本指标进行病理分级.结果胰腺组织的病理损伤严重程度随造模时间的推移逐渐增加,造模后7、14 d大鼠胰腺改变为轻度CP,造模后21、28、35 d大鼠胰腺病理改变为重度CP.重度CP大鼠胰腺组织中甜菜碱(Bet)、胆碱(Cho)化合物含量较轻度CP组及对照组大鼠明显增多,而天冬氨酸盐(Asp)、乳酸(Lac)、脂肪酸(FA)、异亮氨酸/亮氨酸/缬氨酸(Ile/Leu/Val)的含量明显减少.轻度CP与对照组大鼠胰腺组织的代谢产物含量无明显差异.结论应用HR-MAS NMR分析胰腺组织代谢产物有助于区分重度CP与轻度CP.
목적 관찰대서실험성만성이선염(CP)진전과정중이선조직대사산물변화특정,탐토기재CP분급중적개치.방법 Wistar대서36지,경미정맥주입이정기이록화물(dibutyltin dichloride,DBTC)용액8 mg/kg체중제비CP모형.주사후즉각(대조조)급7、14、21、28、35 d분비취대서이선조직,소부분행병리검사,대부분액담냉동후치-80℃보존.응용고분변마각선전핵자공진파보(HRMAS NMR)법검측이선조직적대사산물.이비정상결구、관상복합물、선체위축、섬유화、수종급염세포침윤정도등작위기본지표진행병리분급.결과 이선조직적병리손상엄중정도수조모시간적추이축점증가,조모후7、14 d대서이선개변위경도CP,조모후21、28、35 d대서이선병리개변위중도CP.중도CP대서이선조직중첨채감(Bet)、담감(Cho)화합물함량교경도CP조급대조조대서명현증다,이천동안산염(Asp)、유산(Lac)、지방산(FA)、이량안산/량안산/힐안산(Ile/Leu/Val)적함량명현감소.경도CP여대조조대서이선조직적대사산물함량무명현차이.결론 응용HR-MAS NMR분석이선조직대사산물유조우구분중도CP여경도CP.
Objective To observe the metabolic characteristics of an experimental model of chronic pancreatitis,and to investigate its role in the grading of chronic pancreatitis.Methods Thirty-six Wistar rats were injected with dibutyltin chloride (DBTC) solution (8mg/kg) via the tail vein to establish the experimental model of chronic pancreatitis.The 36 rats were divided into 6 groups with 6 rats in each group.On0,7,14,21,28,35 days after modeling,rats was sacrificed and pancreatic tissue of the rats was harvested,and a small part was used for pathologic study,the majority part was kept at-80℃ under liquid nitrogen freezing.Metabolites of pancreatic tissue were determined by high-resolution magic angle spinning nuclear magnetic resonance spectroscopic (HR-MAS NMRS).On the basis of the abnormal structure,tubular complexes,gland atrophy,fibrosis,edema and inflammatory cell infiltration,chronic pancreatitis was graded.Results Pathologic study showed the severity of chronic pancreatitis gradually increased with time after modeling.The 7th,14th day after modeling,the pancreatic change was mild chronic pancreatitis; the 21st,28th,35th day,the pancreatic change was changed into severe chronic pancreatitis.Principal component analysis of HR-MAS NMRS showed that the betaine (Bet) and choline ( Cho)-contained components were significantly increased in severe chronic pancreatitis; while aspartate (Asp),lactate (Lac),isoleucine/leucine/valine (I/L/V) and fatty acid (FA) were significantly reduced when compared with those in mild chronic pancreatitis and normal pancreatic tissue.There was no significant difference in the amount of metabolic characteristics between mild chronic pancreatitis and normal pancreatic tissue.Conclusions HRMAS NMRS was helpful in distinguishing the severe chronic pancreatitis from mild chronic pancreatitis.