CAJ | 학술논문

目的研究氡吸入染毒小鼠肺及支气管组织中JNK/SAPK信号通路的表达变化.方法采用SR-NIM02型氡室对小鼠进行吸入染毒0.02、30和60工作水平月(WLM)),染毒结束后24 h内处死;提取小鼠肺及支气管组织总RNA,利用Real-Time PCR定量分析JNK mRNA的表达水平;分别利用Western blot和免疫组织化学技术检测肺及支气管组织中JNK蛋白表达及其磷酸化水平.结果 Real-time PCR检测结果显示,染毒30与60 WLM组的JNK mBNA水平分别是对照组的3.56倍和2.96倍(单因素方差分析,F=8.00,P＜0.05);Western blot结果显示,染毒30和60 WLM组的JNK蛋白相对表达量分别为对照组的1.21和1.35倍(单因素方差分析,F=5.76,P＜0.05),JNK蛋白磷酸化水平分别为对照组的2.34和2.81倍(单因素方差分析,F=6.83,P＜0.05);免疫组化结果显示,染毒后小鼠肺组织中JNK与胞核中磷酸化JNK蛋白表达增强.结论氡吸入染毒后可激活细胞内的JNK/SAPK信号通路,使其表达增强,并且磷酸化激活进入胞核,进而引起组织细胞的一系列反应.
목적 연구동흡입염독소서폐급지기관조직중JNK/SAPK신호통로적표체변화.방법 채용SR-NIM02형동실대소서진행흡입염독0.02、30화60공작수평월(WLM)),염독결속후24 h내처사;제취소서폐급지기관조직총RNA,이용Real-Time PCR정량분석JNK mRNA적표체수평;분별이용Western blot화면역조직화학기술검측폐급지기관조직중JNK단백표체급기린산화수평.결과 Real-time PCR검측결과현시,염독30여60 WLM조적JNK mBNA수평분별시대조조적3.56배화2.96배(단인소방차분석,F=8.00,P＜0.05);Western blot결과현시,염독30화60 WLM조적JNK단백상대표체량분별위대조조적1.21화1.35배(단인소방차분석,F=5.76,P＜0.05),JNK단백린산화수평분별위대조조적2.34화2.81배(단인소방차분석,F=6.83,P＜0.05);면역조화결과현시,염독후소서폐조직중JNK여포핵중린산화JNK단백표체증강.결론 동흡입염독후가격활세포내적JNK/SAPK신호통로,사기표체증강,병차린산화격활진입포핵,진이인기조직세포적일계렬반응.
Objective To study the expression of JNK/SAPK(c-Jun NH2-terminal kinase/stress activated protein kinase)in lung and bronchus of radon-exposed mice.Methods Male BALB/c mice were exposed to radon and its progeny with the cumulative dose of 0.02,30 or 60 working level month(WLM),respectively.The expression levels of JNK/SAPK in lung and bronchus were determined with Real-Time PCR,Western blot and immunohistochemistry methods.Results The JNK mRNA levels in lung tissues of mice exposed to radon of 30 and 60 WLM were higher than those of the control by 3.56 and 2.96 times,respectively.The relative expression levels of JNK and phospho-JNK proteins were higher than those of the control by using Western blot and immunohistochemistry methods.Condusiom Expose to the radon and its progeny might activate the JNK/SAPK intracellular signaling pathway.