CAJ | 학술논문

目的探讨霉酚酸(MPA)对人外周髓样树突状细胞(MDC)成熟、吞噬及刺激同种异体CD4+T淋巴细胞增殖能力的影响.方法获得健康志愿者外周血单个核细胞(n=15),实验组加入MPA培养后,流式细胞仪分析MDC表面共刺激因子和黏附分子的表达水平;分离树突细胞抗原-1+(BDCA-1+)细胞,流式细胞仪测定BDCA-1+细胞中FITC所标记的右旋糖酐的荧光值;通过混合淋巴细胞反应,流式细胞仪测定各组CD4+T淋巴细胞在G0期的比例.结果与对照组相比,MPA能明显降低实验组MDC表面CIMO、CD62L、HLA-DR、CD54、CD80、CD83和CD86的表达水平(t=-3.713,P=0.010).MPA能明显增强实验组MDC的吞噬能力(t=10.171,P=0.000).MPA几乎完全抑制实验组MDC诱导CD4+T淋巴细胞分裂、增殖的能力.结论 MPA可增强人外周MDC的吞噬能力,抑制MDC的成熟和抑制MDC刺激同种异体CD4+T淋巴细胞增殖的能力.
목적 탐토매분산(MPA)대인외주수양수돌상세포(MDC)성숙、탄서급자격동충이체CD4+T림파세포증식능력적영향.방법 획득건강지원자외주혈단개핵세포(n=15),실험조가입MPA배양후,류식세포의분석MDC표면공자격인자화점부분자적표체수평;분리수돌세포항원-1+(BDCA-1+)세포,류식세포의측정BDCA-1+세포중FITC소표기적우선당항적형광치;통과혼합림파세포반응,류식세포의측정각조CD4+T림파세포재G0기적비례.결과 여대조조상비,MPA능명현강저실험조MDC표면CIMO、CD62L、HLA-DR、CD54、CD80、CD83화CD86적표체수평(t=-3.713,P=0.010).MPA능명현증강실험조MDC적탄서능력(t=10.171,P=0.000).MPA궤호완전억제실험조MDC유도CD4+T림파세포분렬、증식적능력.결론 MPA가증강인외주MDC적탄서능력,억제MDC적성숙화억제MDC자격동충이체CD4+T림파세포증식적능력.
Objective To study the effect of mycophenolic acid(MPA)on maturation, endocytotic capacity and allostimulatory properties of human myeloid dendritic cell ( MDC ). Methods The peripheral blood mononuclear cells were isolated freshly from healthy volunteers (n = 15). The study group was treated by MPA. Co-stimulatory and adhesion molecular on MDC were analyzed by flow cytometry. After isolation of blood dendritic cell antigen-1 + ( BDCA-1 + ), the cellular uptake of FITC-dextran was measured by flow cytometry. After mixed lymphocyte reaction, the percentage of allogenic CD+4 T cell in G0 phase was analyzed by flow cytometry. Results ( 1) Maturation: Compared to control group, MPA down-regulated the level of CD40, CD62L, HLA-DR, CD54, CD80, CD83 and CD86 on MDC significantly in study group (t = -3. 713,P> 0.010). (2) Endocytotic capacity: MPA enchanced significantly the endocytotic capacity of MDC in study group (t= 10. 171 ,P =0.000). (3) Allostimulatory capacity: Compared to control group, MPA reduced the ability of MDC to stimulate markedly the T cell proliferation in study group. Conclusion MPA may enhance the phagocytic capacity of human peripheral MDC, inhibit the maturity of MDC and impair its allostimulatory capacity of CD+4 T lymphocyte.