中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
6期
403-406
,共4页
武增瀛%杜晓辉%徐迎新%李力%刘巨超%王金晶%李则学
武增瀛%杜曉輝%徐迎新%李力%劉巨超%王金晶%李則學
무증영%두효휘%서영신%리력%류거초%왕금정%리칙학
胃肿瘤%肿瘤%实验性%T淋巴细胞%细胞毒性%杀伤细胞%体层摄影术%发射型计算机%单光子
胃腫瘤%腫瘤%實驗性%T淋巴細胞%細胞毒性%殺傷細胞%體層攝影術%髮射型計算機%單光子
위종류%종류%실험성%T림파세포%세포독성%살상세포%체층섭영술%발사형계산궤%단광자
Stomach neoplasms%Neoplasms,experimental%T-lymphocytes,cytotoxic%Killer cells%Tomography,emission-computed,single-photon
目的 研究细胞毒性T淋巴细胞(CTL)和细胞因子诱导杀伤(CIK)细胞在荷人胃癌裸鼠体内的迁徙与分布特点.方法 采用皮下注射人低分化胃腺癌细胞株BGC-823的方法建立荷人胃癌裸鼠模型.将荷瘤裸鼠分为2组,每组9只,分别经尾静脉输入~(99)Tc~m标记的CTL和CIK细胞.输人后1、6和24 h,每组各取3只裸鼠,应用单光子发射型计算机体层摄影术(SPECT)观察2种细胞在裸鼠体内的分布状况,之后处死动物取肿瘤和肝、脾、肾、肺、肠等器官,以井型探测器测量每克组织百分注入活度(%ID/g),计算肿瘤组织%ID/g与正常组织%ID/g的比值(T/NT).结果 SPECT示踪显示2种细胞输入裸鼠体内后均迅速分布至肿瘤和肝、脾、肾、肺、肠等器官.CTL的%ID/g峰值由高到低依次为肿瘤(7.79±0.46)、肝(4.12±0.51)、肠(2.71±0.16)、肾(1.44±0.25)、脾(1.24±0.12)和肺(1.12±0.11),各组织器官T/NT值均>1;CIK 细胞的%ID峰值由高到低依次为肝(6.64±0.67)、肿瘤(5.47±0.87)、肠(3.55±0.23)、肾(2.34±0.41)、脾(1.45±0.17)和肺(1.27±0.21),除肝以外,其他各组织器官T/NT值均>1.细胞输入后1、6和24 h在肿瘤组织的%ID/g值,CTL分别为2.35±0.28、4.58±0.52和7.79±0.46,CIK 细胞分别为2.23±0.46、3.25±0.70和5.47±0.87,24 h时间点CTL组明显高于CIK组(P<0.05).结论 CTL和CIK细胞在荷瘤裸鼠体内的分布均具有明确的肿瘤靶向性.
目的 研究細胞毒性T淋巴細胞(CTL)和細胞因子誘導殺傷(CIK)細胞在荷人胃癌裸鼠體內的遷徙與分佈特點.方法 採用皮下註射人低分化胃腺癌細胞株BGC-823的方法建立荷人胃癌裸鼠模型.將荷瘤裸鼠分為2組,每組9隻,分彆經尾靜脈輸入~(99)Tc~m標記的CTL和CIK細胞.輸人後1、6和24 h,每組各取3隻裸鼠,應用單光子髮射型計算機體層攝影術(SPECT)觀察2種細胞在裸鼠體內的分佈狀況,之後處死動物取腫瘤和肝、脾、腎、肺、腸等器官,以井型探測器測量每剋組織百分註入活度(%ID/g),計算腫瘤組織%ID/g與正常組織%ID/g的比值(T/NT).結果 SPECT示蹤顯示2種細胞輸入裸鼠體內後均迅速分佈至腫瘤和肝、脾、腎、肺、腸等器官.CTL的%ID/g峰值由高到低依次為腫瘤(7.79±0.46)、肝(4.12±0.51)、腸(2.71±0.16)、腎(1.44±0.25)、脾(1.24±0.12)和肺(1.12±0.11),各組織器官T/NT值均>1;CIK 細胞的%ID峰值由高到低依次為肝(6.64±0.67)、腫瘤(5.47±0.87)、腸(3.55±0.23)、腎(2.34±0.41)、脾(1.45±0.17)和肺(1.27±0.21),除肝以外,其他各組織器官T/NT值均>1.細胞輸入後1、6和24 h在腫瘤組織的%ID/g值,CTL分彆為2.35±0.28、4.58±0.52和7.79±0.46,CIK 細胞分彆為2.23±0.46、3.25±0.70和5.47±0.87,24 h時間點CTL組明顯高于CIK組(P<0.05).結論 CTL和CIK細胞在荷瘤裸鼠體內的分佈均具有明確的腫瘤靶嚮性.
목적 연구세포독성T림파세포(CTL)화세포인자유도살상(CIK)세포재하인위암라서체내적천사여분포특점.방법 채용피하주사인저분화위선암세포주BGC-823적방법건립하인위암라서모형.장하류라서분위2조,매조9지,분별경미정맥수입~(99)Tc~m표기적CTL화CIK세포.수인후1、6화24 h,매조각취3지라서,응용단광자발사형계산궤체층섭영술(SPECT)관찰2충세포재라서체내적분포상황,지후처사동물취종류화간、비、신、폐、장등기관,이정형탐측기측량매극조직백분주입활도(%ID/g),계산종류조직%ID/g여정상조직%ID/g적비치(T/NT).결과 SPECT시종현시2충세포수입라서체내후균신속분포지종류화간、비、신、폐、장등기관.CTL적%ID/g봉치유고도저의차위종류(7.79±0.46)、간(4.12±0.51)、장(2.71±0.16)、신(1.44±0.25)、비(1.24±0.12)화폐(1.12±0.11),각조직기관T/NT치균>1;CIK 세포적%ID봉치유고도저의차위간(6.64±0.67)、종류(5.47±0.87)、장(3.55±0.23)、신(2.34±0.41)、비(1.45±0.17)화폐(1.27±0.21),제간이외,기타각조직기관T/NT치균>1.세포수입후1、6화24 h재종류조직적%ID/g치,CTL분별위2.35±0.28、4.58±0.52화7.79±0.46,CIK 세포분별위2.23±0.46、3.25±0.70화5.47±0.87,24 h시간점CTL조명현고우CIK조(P<0.05).결론 CTL화CIK세포재하류라서체내적분포균구유명학적종류파향성.
Objective To investigate the migration and distribution of CTL (cytotoxic T lymphocyte) and CIK (cytokine-induced killer) cells in gastric tumor model. Methods Subcutaneous gastric tumor model was established by BGC-823 cancer cells in nude mice. Both CTL and CIK cells were labeled with~(99)Tc~m directly and then inoculated into nude mice with subcutaneous tumor by intravenous injection separately. Three mice of each group were evaluated by single-photon emission computerized tomography (SPECT) at 1 h, 6 h and 24 h post-inoculation. After SPECT imaging,3 mice in each group were sacrificed and got samples of the tumor, liver, spleen, kidney, lung, intestine, etc. The tissue samples were weighed and radioactivity was determined with a well-type scintillation counter. The accumulation of labeled CTL and CIK cells in tissues were expressed as % ID/g (percentage activity of injection dose per gram of tissue) and T/NT (tumor/non-tumor) values were analyzed. Results The tracing of both cells in SPECT showed a clear migration path away from the injection point to solid tumor, and can be detected in all organs and tissues such as liver, spleen, kidney, lung and intestine, etc not long after injection. The % ID/g peak values of CTL in organs from the highest to the lowest were as follows: tumor (7.79±0.46), liver (4.12±0.51), intestine (2.71±0.16), kidney (1.44±0.25), spleen (1.24±0.12), kidney (1.12±0.11), and all the T/NTs were above 1. The % ID/g peak values of CIK cells in organs from the highest to the lowest were as follows: liver (6.64±0.67), tumor (5.47±0.87), intestine (3.55±0.23), kidney (2.34±0.41), spleen (1.45±0.17), lung (1.27±0.21), and T/NTs>1 except for liver. After injection, the % ID/g values of tumor in CTL group were 2.35±0.28 (1 h), 4.58±0.52 (6 h) and 7.79±0.46 (24 h) respectively while the % ID/g values of tumor in CIK group 2.23±0.46 (1 h), 3.25±0.70 (6 h) and 5.47±0.87 (24 h) respectively. At 24 h point, the % ID/g of CTL in tumor was much higher than CIK cells (P<0.05). Conclusion The definite directional tumor-targeting capacity of CTL and CIK cells in tumor-bearing nude mice is promising.
