防护林科技
防護林科技
방호림과기
PROTECTION FOREST SCIENCE AND TECHNOLOGY
2002年
4期
29-31
,共3页
褚建民%周凌娟%王阳%王东启%商政义
褚建民%週凌娟%王暘%王東啟%商政義
저건민%주릉연%왕양%왕동계%상정의
白玉兰%离体培养%快速繁殖
白玉蘭%離體培養%快速繁殖
백옥란%리체배양%쾌속번식
试验建立了白玉兰离体繁殖体系.种子为外植体,初代培养基为:B5+BA0.5 mg/L+NAA0.05 mg/L+30g/L糖+琼脂6g/L,萌发率85%.继代培养基为:B5+BA1.5 mg/L+NAA0.15mg/L+30g/glucose/sucrose+6g/L agar+Vco0.5g/L,月增殖率可达5.0±0.1.生根培养为:1/2B5+NAA0.5 mg/L+AC1.0 mg/L+VB20 mg/L.以试管苗茎段、根段做外植体,培养基B5+BA2 mg/L+2.4-D0.5 mg/L+VC0.5g/L,可获得少量愈伤组织,继代存活率仅为30%.没有再分化成苗.
試驗建立瞭白玉蘭離體繁殖體繫.種子為外植體,初代培養基為:B5+BA0.5 mg/L+NAA0.05 mg/L+30g/L糖+瓊脂6g/L,萌髮率85%.繼代培養基為:B5+BA1.5 mg/L+NAA0.15mg/L+30g/glucose/sucrose+6g/L agar+Vco0.5g/L,月增殖率可達5.0±0.1.生根培養為:1/2B5+NAA0.5 mg/L+AC1.0 mg/L+VB20 mg/L.以試管苗莖段、根段做外植體,培養基B5+BA2 mg/L+2.4-D0.5 mg/L+VC0.5g/L,可穫得少量愈傷組織,繼代存活率僅為30%.沒有再分化成苗.
시험건립료백옥란리체번식체계.충자위외식체,초대배양기위:B5+BA0.5 mg/L+NAA0.05 mg/L+30g/L당+경지6g/L,맹발솔85%.계대배양기위:B5+BA1.5 mg/L+NAA0.15mg/L+30g/glucose/sucrose+6g/L agar+Vco0.5g/L,월증식솔가체5.0±0.1.생근배양위:1/2B5+NAA0.5 mg/L+AC1.0 mg/L+VB20 mg/L.이시관묘경단、근단주외식체,배양기B5+BA2 mg/L+2.4-D0.5 mg/L+VC0.5g/L,가획득소량유상조직,계대존활솔부위30%.몰유재분화성묘.
