CAJ | 학술논문

目的:探讨小鼠肾间质纤维化早期是否存在肾小管上皮细胞间充质细胞转型的形态学变化.方法:利用单侧输尿管梗阻的方法建立小鼠肾间质纤维化模型,常规组织学分析小鼠肾脏病理变化,免疫组化检测a-SMA的表达,肾基底膜六胺银染色和透射电镜观察肾小管基底膜的变化情况,明胶酶谱分析观察肾脏中MMP2和MMP9的表达情况.结果:成功地建立了小鼠肾间质纤维化模型,并被组织学分析证实.免疫组化分析显示肾脏中出现了大量表达a-SMA的细胞,肾基底膜六胺银染色和透射电镜分析显示小鼠肾间质纤维化早期肾脏出现了肾小管基底膜局部断裂和肾小管上皮细胞迁出的改变,明胶酶谱分析显示小鼠肾脏中MMP2和MMP9于肾间质纤维化早期有一过性增高变化.结论:肾小管基底膜局部断裂,肾小管上皮细胞迁出以及MMP2和MMP9的表达变化参与了小鼠肾间质纤维化早期上皮细胞-间充质细胞转型的过程.
목적:탐토소서신간질섬유화조기시부존재신소관상피세포간충질세포전형적형태학변화.방법:이용단측수뇨관경조적방법건립소서신간질섬유화모형,상규조직학분석소서신장병리변화,면역조화검측a-SMA적표체,신기저막륙알은염색화투사전경관찰신소관기저막적변화정황,명효매보분석관찰신장중MMP2화MMP9적표체정황.결과:성공지건립료소서신간질섬유화모형,병피조직학분석증실.면역조화분석현시신장중출현료대량표체a-SMA적세포,신기저막륙알은염색화투사전경분석현시소서신간질섬유화조기신장출현료신소관기저막국부단렬화신소관상피세포천출적개변,명효매보분석현시소서신장중MMP2화MMP9우신간질섬유화조기유일과성증고변화.결론:신소관기저막국부단렬,신소관상피세포천출이급MMP2화MMP9적표체변화삼여료소서신간질섬유화조기상피세포-간충질세포전형적과정.
Objective: To observe morphological changes of epithelial-mesenchymal transition in the early stage of mice renal interstitial fibrosis. Methods: Renal interstitial fibrosis was induced by unilateral ureteral obstruction(UUO) in mice. Histological and immunohistochemical methods were used to analyze pathological changes and α-SMA expression in renal tissue.Argentum hexamethylenamine staining and transmission electron microscopy were used to observe changes of the renal tubule basement membrane. Gelatin zymographic analysis was used to observe the expression of MMP2 and MMP9 in renal tissue.Results:The mice suffered from renal interstitial fibrosis were identified by histological analysis and α-SMA positive cells in renal tissue. Argentum hexamethylenamine staining and transmission electron-microscopy showed that the renal tubule basement membrane disrupted locally and renal tubule epithelial cells invaded into the renal interstitium in the early stage of renal interstitial fibrosis. Gelatin zymographic analysis showed that the expression of MMP2 and MMP9 was increased transitorily in the early stage of renal interstitial fibrosis. Conclusion: Renal tubule basement membrane disruption, renal tubule epithelial cells invasion into the renal interstitium, and the expression of MMP2 and MMP9 are involved in the development of renal interstitial fibrosis.