CAJ | 학술논문

系统采集中国沿海10种代表性海藻,进行有效成分提取与粗分;利用昆虫杆状病毒表达系统克隆人COX-2基因,并在昆虫Spodoptera frugiperda(sf9)细胞中表达获得COX-2蛋白;以花生四烯酸为底物,通过测定前列腺素PGE2的生成浓度,测定海藻不同部位对COX-2酶的抑制活性.结果表明,萱藻(Scytosiphon lomentarius)乙醇提取物、鼠尾藻(Sargassum thunbergii)和刺松藻(Codium fragile)乙酸乙酯相、松节藻(Rhodomela conferroides)正丁醇相表现出良好的COX-2酶抑制活性(质量浓度为10μg/mL时,抑制率大于50%).首次对上述海藻进行COX-2酶抑制活性研究,其中萱藻乙醇提取物、鼠尾藻和刺松藻乙酸乙酯相、松节藻正丁醇相表现出良好的COX-2抑制活性.
계통채집중국연해10충대표성해조,진행유효성분제취여조분;이용곤충간상병독표체계통극륭인COX-2기인,병재곤충Spodoptera frugiperda(sf9)세포중표체획득COX-2단백;이화생사희산위저물,통과측정전렬선소PGE2적생성농도,측정해조불동부위대COX-2매적억제활성.결과표명,훤조(Scytosiphon lomentarius)을순제취물、서미조(Sargassum thunbergii)화자송조(Codium fragile)을산을지상、송절조(Rhodomela conferroides)정정순상표현출량호적COX-2매억제활성(질량농도위10μg/mL시,억제솔대우50%).수차대상술해조진행COX-2매억제활성연구,기중훤조을순제취물、서미조화자송조을산을지상、송절조정정순상표현출량호적COX-2억제활성.
Cyclooxygenase-2 (COX-2) is a key enzyme that catalyzes the biosynthesis of prostaglandins from arachidonic acid and plays a critical role in inflammation, pain and fever. In order to search for new type inhibitors against COX-2, extractions from 10 algae were screened indirectly by determination of prostaglandin E2 which was synthesized from arachidonic acid. Human cyclooxygenase-2 genes were cloned from human monocyte cell line THP-1 cells and expressed in Spodoptera frugiperda (sf9) insect cell line by Bac-to-Bac baculovirus expression systems. The results showed that ethanolic extraction of Scytosiphon lomentarius, EtOAc phase of Sargassum thunbergii and Codium fragile and butanolic phase of Rhodomela confervoides exhibited good bioactivities with inhibitory ratio higher than 50% at a dose of 10 μg/mL.