CAJ | 학술논문

目的研究大鼠延髓内脏带(MVZ)内星形胶质细胞与神经元对选择性性神经病理性痛的反应.方法 25只成年 SD 大鼠,其中15只为接受左侧坐骨神经分支选择性损伤(SNI)组,5只作为假手术组,5只为对照组.在术前、术后10d、20d、30d(每时段5只)观察大鼠疼痛行为学并检测机械刺激缩足反射阈值(PWMT)的变化;应用抗Fos蛋白与抗胶质原纤维酸性蛋白(GFAP)或抗Fos蛋白与抗酪氨酸羟化酶(TH)的单一或双重免疫荧光法染色,Confocal显微镜下观察延髓MVZ内GFAP标记的星形胶质细胞的荧光强度,Fos/ GFAP双标记星形胶质细胞以及Fos/TH双标记神经元的平均数. 结果 SNI组大鼠与对照组或假手术组相比,术后10d其痛敏增高(PWMT值降低),20d痛敏达到高峰(PWMT值最低).免疫荧光染色显示:SNI术后MVZ内星形胶质细胞表现为激活型,GFAP免疫荧光强度明显增加;孤束核及腹外侧区的Fos/GFAP双标记星形胶质细胞及Fos/TH双标记神经元的平均值显著增高,SNI术后20d达到高峰. 结论 SNI术后MVZ内的星形胶质细胞和神经元均被激活.
목적 연구대서연수내장대(MVZ)내성형효질세포여신경원대선택성성신경병이성통적반응.방법 25지성년 SD 대서,기중15지위접수좌측좌골신경분지선택성손상(SNI)조,5지작위가수술조,5지위대조조.재술전、술후10d、20d、30d(매시단5지)관찰대서동통행위학병검측궤계자격축족반사역치(PWMT)적변화;응용항Fos단백여항효질원섬유산성단백(GFAP)혹항Fos단백여항락안산간화매(TH)적단일혹쌍중면역형광법염색,Confocal현미경하관찰연수MVZ내GFAP표기적성형효질세포적형광강도,Fos/ GFAP쌍표기성형효질세포이급Fos/TH쌍표기신경원적평균수. 결과 SNI조대서여대조조혹가수술조상비,술후10d기통민증고(PWMT치강저),20d통민체도고봉(PWMT치최저).면역형광염색현시:SNI술후MVZ내성형효질세포표현위격활형,GFAP면역형광강도명현증가;고속핵급복외측구적Fos/GFAP쌍표기성형효질세포급Fos/TH쌍표기신경원적평균치현저증고,SNI술후20d체도고봉. 결론 SNI술후MVZ내적성형효질세포화신경원균피격활.
Objective To investigate the response of astrocytes and neurons in the medullary visceral zone (MVZ) to neuropathic pain induced by the spared nerve injury (SNI, the tibial nerve and the common peroneal nerve were sectioned, while the sural nerve was intacted). Methods The SNI operation and sham operation (only incised the skin of thigh, but the tibial nerve and the common peroneal nerve did not cut) were performed in adult male Sprague-Dawley rats. The paw withdrawal mechanical threshold (PWMT) was measured at 10,20,30 days after operation. By using single or double immunofluorescent staining method,we investigated and measured that the mean fluorescent intensity (MFI) of anti-glial fibrillary acidic protein (GFAP, a marker of astrocytes) signal in the astrocytes, the mean member of Fos/ GFAP double labeled astrocytes and Fos / tyrosine hydorxylase (TH) double labeled neurons in MVZ after operation. Results As compared with control or sham groups, SNI induced that the PWMT became significant sensitivity and peaked at 20 days after SNI. The activated astrocytes revealed an activated morphology, the MFI of anti-GFAP signal markedly strengthened, the mean number of Fos/GFAP double labeled astrocytes and Fos/TH double labeled neurons in the nucleus of the tract solitarius (NTS) and the ventral lateral medulla (VLM) increased significantly and peaked at 20 days after SNI. Conclusion The neurons and astrocytes in MVZ were sensitively activated by SNI.