CAJ | 학술논문

对羊栖菜(Hizikia fusiformis (Harv) Okamura)养殖中常见的3个品系进行了DNA指纹分析及遗传变异的研究,构建了其遗传指纹图谱,分析了不同种群的遗传关系,为羊栖菜的种质鉴定及选育提供了理论依据.运用RAPD分子标记技术,对5个羊栖菜的种群中共125个个体进行了分析,从300个引物中筛选出12条随机扩增引物共扩增135个位点,多态位点比率为84.4%.从中选择了4个多态性位点,构建了DNA指纹图谱.相关结果对羊栖菜遗传选育和种质鉴定等有参考价值.
대양서채(Hizikia fusiformis (Harv) Okamura)양식중상견적3개품계진행료DNA지문분석급유전변이적연구,구건료기유전지문도보,분석료불동충군적유전관계,위양서채적충질감정급선육제공료이론의거.운용RAPD분자표기기술,대5개양서채적충군중공125개개체진행료분석,종300개인물중사선출12조수궤확증인물공확증135개위점,다태위점비솔위84.4%.종중선택료4개다태성위점,구건료DNA지문도보.상관결과대양서채유전선육화충질감정등유삼고개치.
In this study, we analyzed the genetic distances and constructed the fingerprints of the three good strains, which have been widely applied in the production. It was indicated that: RAPD (random amplified polymorphic DNA) was applied to analyzing 125 individuals from the 5 populations of Hizikia. A total of 135 amplification loci were obtained from 12 selected primers after screening and P% (the percentage of polymorphic loci) is 84.4%. The yielded 4 stable and clear bands were selected to construct a fingerprint for discrimination of the 5 populations. Our results provide the tools for the Hizikias genetic breeding and germplasm identification in the future.