天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2010年
3期
471-474
,共4页
金线莲%黄酮类%球面设计%糖耐受
金線蓮%黃酮類%毬麵設計%糖耐受
금선련%황동류%구면설계%당내수
Anoectochilus roxburghii%flavonoids%spherical symmetric design%glucose tolerance
以金线莲为材料,利用统计学实验设计方法对黄酮类物质的提取工艺进行了优化.选定液料比、乙醇浓度和时间三个因素的五个水平进行球面设计实验,建立黄酮提取率的二次回归方程,通过回归分析及岭脊分析得到优化提取组合条件.研究结果表明,当提取工艺条件为:液料比24,乙醇浓度63%,提取时间48 h时,黄酮提取率的最大预测值为1.107%,验证值为1.05%.葡萄糖耐受性试验结果表明:金线莲黄酮类萃取物能够提高正常小鼠对糖的耐受性.
以金線蓮為材料,利用統計學實驗設計方法對黃酮類物質的提取工藝進行瞭優化.選定液料比、乙醇濃度和時間三箇因素的五箇水平進行毬麵設計實驗,建立黃酮提取率的二次迴歸方程,通過迴歸分析及嶺脊分析得到優化提取組閤條件.研究結果錶明,噹提取工藝條件為:液料比24,乙醇濃度63%,提取時間48 h時,黃酮提取率的最大預測值為1.107%,驗證值為1.05%.葡萄糖耐受性試驗結果錶明:金線蓮黃酮類萃取物能夠提高正常小鼠對糖的耐受性.
이금선련위재료,이용통계학실험설계방법대황동류물질적제취공예진행료우화.선정액료비、을순농도화시간삼개인소적오개수평진행구면설계실험,건립황동제취솔적이차회귀방정,통과회귀분석급령척분석득도우화제취조합조건.연구결과표명,당제취공예조건위:액료비24,을순농도63%,제취시간48 h시,황동제취솔적최대예측치위1.107%,험증치위1.05%.포도당내수성시험결과표명:금선련황동류췌취물능구제고정상소서대당적내수성.
The extraction of flavonoids from Anoectochilus roxburghii was studied in this paper. The effect of the extraction parameters of extraction time,concentration and ratio of ethanol to raw material were optimized by using statistical techniques such as a three factor,five level spherical symmetric design-response surface methodology,with a view to maximize the extraction yield under optimal extraction parameters combination. From this study,it could be concluded that the maximum yield of the tlavonoids ( 1.05 % ) can be obtained ethanol concentration of 63.02% ,extraction time of 47.9 h,ratio of water to material of 24.25:1. Glucose tolerance test show that flavonoid-riched from Anoectochilus roxburghil extract can improve the sugar tolerance of normal mice.
