CAJ | 학술논문

[目的]论证线粒体Cyt b 和12S rRNA基因不同进化速率影响构建的系统树拓扑结构.[方法]从GenBank下载了蛇亚目12科15种蛇线粒体全序列,提取Cyt b 和12S rRNA基因序列,选用GTR+I+G核苷酸替代模型,基于最大似然法,用PAUP4.0软件分别构建2个基因的系统关系树.[结果]在选用相同的软件、建树方法和研究物种的情况下,构建的系统树拓扑结构差异主要是因为线粒体Cyt b 和12S rRNA基因进化速率不同产生的.[结论]在分子系统进化研究中,要针对不同的研究物种和研究目的,选择合适的基因构建系统进化树.
[목적]론증선립체Cyt b 화12S rRNA기인불동진화속솔영향구건적계통수탁복결구.[방법]종GenBank하재료사아목12과15충사선립체전서렬,제취Cyt b 화12S rRNA기인서렬,선용GTR+I+G핵감산체대모형,기우최대사연법,용PAUP4.0연건분별구건2개기인적계통관계수.[결과]재선용상동적연건、건수방법화연구물충적정황하,구건적계통수탁복결구차이주요시인위선립체Cyt b 화12S rRNA기인진화속솔불동산생적.[결론]재분자계통진화연구중,요침대불동적연구물충화연구목적,선택합괄적기인구건계통진화수.
[Objective] The aim was to prove that the mitochondrial genes of Cyt b and 12S rRNA with different evolutional rates have effects on the topological structures of phylogenetic trees. [Method] The complete sequences of Cyt b and 12S rRNA from 15 species in 12 families of snakes were downloaded and extracted from GenBank, while their molecular phylogenetic trees were constructed by Maximum Likelihood (ML) method with GTR+I+G substitute model based on PAUP4.0 software. [Result] With the same software, methods and species, the difference in topological structures of phylogenetic trees was mainly due to different evolutional rates of Cytb and 12S rRNA genes. [Conclusion] In studies on phylogenetic trees, aimed to different research species and purposes, phylogenetic trees should be constructed by choosing the correct and appropriate genes.