中华危重症医学杂志（电子版）
中華危重癥醫學雜誌（電子版）
중화위중증의학잡지（전자판）
CHINESE JOURNAL OF CRITICAL CARE MEDICINE ( ELECTRONIC EDITON)
2012年
3期
11-13
,共3页
姚峰%张琴%姜久昆%陆远强
姚峰%張琴%薑久昆%陸遠彊
요봉%장금%강구곤%륙원강
奈瑟球菌,淋病%衣原体,沙眼%解脲支原体%单纯疱疹病毒属%聚合酶链反应
奈瑟毬菌,淋病%衣原體,沙眼%解脲支原體%單純皰疹病毒屬%聚閤酶鏈反應
내슬구균,임병%의원체,사안%해뇨지원체%단순포진병독속%취합매련반응
Neisseria gonorrhoeae%Chlamydia trachomatis%Ureaplasma urealyticum%Simplexvirus%Polymerase chain reaction
目的 建立同时检测淋球菌、沙眼衣原体、解脲支原体和单纯疱疹病毒-2致病微生物的多重聚合酶链反应(PCR)方法,用于四种性病的诊断和治疗监测.方法 根据淋球菌外膜孔蛋白基因、沙眼衣原体基因、解脲支原体尿素酶基因和单纯疱疹病毒-2的DNA聚合酶基因序列,各设计一对特异性引物,优化反应体系和条件进行多重PCR反应.结果 4对引物分别扩增出327、167、426、391 bp的目的条带,并且特异性强,与其他非目的病原体基因不发生反应.结论 本研究建立的多重PCR方法为相关病原体的快速检测提供方法.
目的 建立同時檢測淋毬菌、沙眼衣原體、解脲支原體和單純皰疹病毒-2緻病微生物的多重聚閤酶鏈反應(PCR)方法,用于四種性病的診斷和治療鑑測.方法 根據淋毬菌外膜孔蛋白基因、沙眼衣原體基因、解脲支原體尿素酶基因和單純皰疹病毒-2的DNA聚閤酶基因序列,各設計一對特異性引物,優化反應體繫和條件進行多重PCR反應.結果 4對引物分彆擴增齣327、167、426、391 bp的目的條帶,併且特異性彊,與其他非目的病原體基因不髮生反應.結論 本研究建立的多重PCR方法為相關病原體的快速檢測提供方法.
목적 건립동시검측림구균、사안의원체、해뇨지원체화단순포진병독-2치병미생물적다중취합매련반응(PCR)방법,용우사충성병적진단화치료감측.방법 근거림구균외막공단백기인、사안의원체기인、해뇨지원체뇨소매기인화단순포진병독-2적DNA취합매기인서렬,각설계일대특이성인물,우화반응체계화조건진행다중PCR반응.결과 4대인물분별확증출327、167、426、391 bp적목적조대,병차특이성강,여기타비목적병원체기인불발생반응.결론 본연구건립적다중PCR방법위상관병원체적쾌속검측제공방법.
Objective To develop a multiplex polymerase chain reaction (PCR) method for simultaneous detection of sexually transmitted pathogens.Methods According to specific gene sequence of four pathogens:porin protein of neisseria gonorrhoeae,chlamydia trachomatis,urease of ureaplasma urealyticum and DNA polymerase of human herpesvirus 2,four sets of specific primers were designed and a multiplex PCR assay was developed to detect 4 pathogens in one test based on the optimized reaction system and condition.Results The target DNA at 327,167,426 and 391 bp of 4 pathogens were specifically amplified by their specific primers.The DNA of other several common pathogens including gardnerella vaginalis,trichononiasis,acinetobacter baumannii was not amplified by four sets of primers.Conclusion It was shown that a simultaneous,specific and rapid multiplex PCR assay method was successfully constructed to detect four sexually transmitted pathogens.
