CAJ | 학술논문

目的探讨两种不同微囊化肝细胞包裹技术移植后对急性肝衰鼠的治疗作用.方法建立急性肝衰大鼠模型,分离纯化SD大鼠肝细胞,应用两种不同的微囊化包裹肝细胞方法 I组(Ba2+-Alg)和Ⅱ组(Alg-P11-Alg),移植后观察其模型鼠肝功能生化指标和存活率.结果对照组48 h内存活率仅15.4%,I组和Ⅱ组存活率在96 h后始终维持在60%左右.两组对比无显著性差异,并且肝功能生化指标两组对比亦无显著性差异.结论两种不同微囊化肝细胞包裹方法 ,移植后对急性肝衰鼠具有相同的治疗效果.
목적 탐토량충불동미낭화간세포포과기술이식후대급성간쇠서적치료작용.방법 건립급성간쇠대서모형,분리순화SD대서간세포,응용량충불동적미낭화포과간세포방법 I조(Ba2+-Alg)화Ⅱ조(Alg-P11-Alg),이식후관찰기모형서간공능생화지표화존활솔.결과 대조조48 h내존활솔부15.4%,I조화Ⅱ조존활솔재96 h후시종유지재60%좌우.량조대비무현저성차이,병차간공능생화지표량조대비역무현저성차이.결론 량충불동미낭화간세포포과방법 ,이식후대급성간쇠서구유상동적치료효과.
Objective To investigate the therapeutic effect of two different microencapsulated hepatocytes(Ba2+ -Alginate, Alg-PII-Alg) on rats with acute liver failure(ALF).Methods Microen-capsulated hepatocytes(Ba2+-Alginate and Alg-P11-Alg)harvested in SD rats were transplanted into the peritoneum of Wister rats with ALF induced by 90%liver resection. The survival rate of the rats and variety of biochemistry for microencapsulated hepatocytes were observed. Results In control group transplanted with empty microcaDsulation.the survival rate was 15.4%after 90%liver resettion in 48 h. The survival rate was decreased to 60% in Ba2+ -Alginate group and in Alg-Pll-Alg group after 48 h. Conclusion Two different microencacapsulation methods are able to prevent death in this model of acute liver failure.There is the same therapeutic effect in microencapsulation of the hepato-cytes transplantation. Biochemical studies shows that Ba2+ -Alginate group and Alg-Pll-Alg group are not significantly different.