CAJ | 학술논문

目的探讨T-bet、GATA-3在玫瑰糠疹发病机制中的意义.方法 SYBR Green Ⅰ实时荧光定量PCR技术检测17例玫瑰糠疹患者和20例正常人外周血单一核细胞中T-bet mRNA和GATA-3mRNA的表达.结果玫瑰糠疹患者外周血中T-bet mRNA与GATA-3 mRNA Avg△Ct值分别为3.33±0.94与5.22±0.69,对照组分别为4.31±1.11与4.36±1.02.患者的T-bet mRNA表达高于对照组(P＜0.05),GATA-3表达低于对照组(P＜0.05).结论玫瑰糠疹患者外周血中T-bet/GATA-3升高,Th1优势表达,细胞免疫异常在玫瑰糠疹发病过程中可能具有重要作用.
목적 탐토T-bet、GATA-3재매괴강진발병궤제중적의의.방법 SYBR Green Ⅰ실시형광정량PCR기술검측17례매괴강진환자화20례정상인외주혈단일핵세포중T-bet mRNA화GATA-3mRNA적표체.결과 매괴강진환자외주혈중T-bet mRNA여GATA-3 mRNA Avg△Ct치분별위3.33±0.94여5.22±0.69,대조조분별위4.31±1.11여4.36±1.02.환자적T-bet mRNA표체고우대조조(P＜0.05),GATA-3표체저우대조조(P＜0.05).결론 매괴강진환자외주혈중T-bet/GATA-3승고,Th1우세표체,세포면역이상재매괴강진발병과정중가능구유중요작용.
Objective To investigate the significance of T-bet and GATA-3 in the pathogenesis of pityriasis rosea.Methods SYBR Green Ⅰ real-time fluorescence quantitative PCR was performed to detect the expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells (PBMCs) from 17 patients with pityriasis rosea and 20 normal human controls.Results The average delta Ct value of T-bet mRNA and GATA-3 mRNA was 3.33 ± 0.94 and 5.22 ± 0.69 respectively in the patients,4.31 ± 1.11 and 4.36 ± 1.02respectively in the controls.There was a higher expression of T-bet mRNA but a lower expression of GATA-3 mRNA in the patients compared with the controls (both P ＜ 0.05).Conclusions Patients with pityriasis rosea show an increased ratio of T-bet to GATA3 expression and predominant expression of Th1-type cytokines.Abnormal cellular immunity may play an important role in the pathogenesis of pityriasis rosea.