CAJ | 학술논문

目的评价CYP3A4* 1G基因多态性对女性健康志愿者芬太尼药效学的影响.方法女性健康志愿者28名,年龄18～25岁,身高155 ～ 175.cm,体重45～ 70 kg.采用聚合酶链反应-限制性片段长度多态性技术进行CYP3A4* 1G基因多态性位点的检测.按照基因型分为3组:野生型纯合子组、突变型杂合子组和突变型纯合子组.经1 min静脉注射芬太尼5μg/kg.于给药前和给药后45、150、240 min时,采用电刺激法测定痛阈.结果与野生型纯合子组和突变型杂合子组比较,突变型纯合子组给药后45和150 min痛阈升高(P＜0.05)；野生型纯合子组和突变型杂合子组各时点痛阈比较差异无统计学意义(P＞0.05).结论 CYP3A4* 1G基因突变可增强静脉注射芬太尼的镇痛效应.
목적 평개CYP3A4* 1G기인다태성대녀성건강지원자분태니약효학적영향.방법 녀성건강지원자28명,년령18～25세,신고155 ～ 175.cm,체중45～ 70 kg.채용취합매련반응-한제성편단장도다태성기술진행CYP3A4* 1G기인다태성위점적검측.안조기인형분위3조:야생형순합자조、돌변형잡합자조화돌변형순합자조.경1 min정맥주사분태니5μg/kg.우급약전화급약후45、150、240 min시,채용전자격법측정통역.결과 여야생형순합자조화돌변형잡합자조비교,돌변형순합자조급약후45화150 min통역승고(P＜0.05)；야생형순합자조화돌변형잡합자조각시점통역비교차이무통계학의의(P＞0.05).결론 CYP3A4* 1G기인돌변가증강정맥주사분태니적진통효응.
Objective To investigate the effects of CYP3A4* 1G genetic polymorphism on fentanyl pharmadynamics after intravenous injection in healthy female velunteers,Methods Twenty-eight healthy female volunteers aged 18-25 yr weighing 45-70 kg were enrolled in this study.The CYP3A4 * 1G genetic polymorphic sites were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The volunteers were assigned into 3 groups according to their genotypes:group Ⅰ wild homozygote ; group Ⅱ mutation heterozygote and group Ⅲ mutation homozygote.Fentanyl 5 μg/kg was injected iv over 1 min.Pain threshold was measured using electrical stimulation before and at 45,150 and 240 min after fentanyl injection.Results Pain threshold was significantly higher at 45 and 150 min after iv fentanyl injection in mutation homozygote group than in mutation heterozygote group and wild homozygote group.There was no significant difference in pain threshold between mutation heterozygote group and wild homozygote group.Conclusion CYP3A4* 1G genetic mutation can enhance the analgesic efficacy of fentanyl after intravenous injection in healthy female volunteers.