中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2011年
6期
602-607
,共6页
傅见英%姜葵%张茂俊%何利华%张建中
傅見英%薑葵%張茂俊%何利華%張建中
부견영%강규%장무준%하리화%장건중
幽门螺杆菌%vacA等位基因多态性%分子流行病学
幽門螺桿菌%vacA等位基因多態性%分子流行病學
유문라간균%vacA등위기인다태성%분자류행병학
Helicobacter pylori%vacA allele polymorphism%Molecular epidemiology
目的 分析幽门螺杆菌(HP)中国菌株vacA基因多态性.方法 对分离自中国7个不同地区、不同胃十二指肠相关疾病患者的119株HP,采用特异引物聚合酶链反应(PER)方法,对其vacA基因进行PCR扩增.根据核酸电泳中产物片段大小确定vacA等位基因类型并统计分析各型分布.对vacA基因核心片段进行PCR扩增和DNA测序,利用软件MEGA4.0对DNA测序结果进行聚类分析.结果 119株HP的vacA基因以sla、m2和il型为主,分别为97.5%(116/119)、68.9%(82/119)和91.6%(109/119).26.1%(31/119)为mlb;slb,mla未检出.vacA组合基因型以sla/m2/il为主(62.2%,74/119),sla/mlb/il次之(25.2%,30/119).不同地区、不同疾病来源菌株sla分布的差异无统计学意义(P>005).而m区基因多态性在疾病类型及分离地区间差异有统计学意义(P<0.01).不同疾病来源菌株间i区基因分型分布的差异无统计学意义,但不同分离地区菌株间差异有统计学意义(P<0.01).119株的vacA基因序列聚类为三个不同组群.结论 HP中国分离株vacA基因型以sla/m2/il组合型为主,sla分型结果与菌株分离地区及疾病类型无关.不同地区、不同疾病来源菌株m区基因分型不同.不同地区菌株vacA基因i区分型不同,但i区基因分型与菌株的疾病来源无显著相关性.
目的 分析幽門螺桿菌(HP)中國菌株vacA基因多態性.方法 對分離自中國7箇不同地區、不同胃十二指腸相關疾病患者的119株HP,採用特異引物聚閤酶鏈反應(PER)方法,對其vacA基因進行PCR擴增.根據覈痠電泳中產物片段大小確定vacA等位基因類型併統計分析各型分佈.對vacA基因覈心片段進行PCR擴增和DNA測序,利用軟件MEGA4.0對DNA測序結果進行聚類分析.結果 119株HP的vacA基因以sla、m2和il型為主,分彆為97.5%(116/119)、68.9%(82/119)和91.6%(109/119).26.1%(31/119)為mlb;slb,mla未檢齣.vacA組閤基因型以sla/m2/il為主(62.2%,74/119),sla/mlb/il次之(25.2%,30/119).不同地區、不同疾病來源菌株sla分佈的差異無統計學意義(P>005).而m區基因多態性在疾病類型及分離地區間差異有統計學意義(P<0.01).不同疾病來源菌株間i區基因分型分佈的差異無統計學意義,但不同分離地區菌株間差異有統計學意義(P<0.01).119株的vacA基因序列聚類為三箇不同組群.結論 HP中國分離株vacA基因型以sla/m2/il組閤型為主,sla分型結果與菌株分離地區及疾病類型無關.不同地區、不同疾病來源菌株m區基因分型不同.不同地區菌株vacA基因i區分型不同,但i區基因分型與菌株的疾病來源無顯著相關性.
목적 분석유문라간균(HP)중국균주vacA기인다태성.방법 대분리자중국7개불동지구、불동위십이지장상관질병환자적119주HP,채용특이인물취합매련반응(PER)방법,대기vacA기인진행PCR확증.근거핵산전영중산물편단대소학정vacA등위기인류형병통계분석각형분포.대vacA기인핵심편단진행PCR확증화DNA측서,이용연건MEGA4.0대DNA측서결과진행취류분석.결과 119주HP적vacA기인이sla、m2화il형위주,분별위97.5%(116/119)、68.9%(82/119)화91.6%(109/119).26.1%(31/119)위mlb;slb,mla미검출.vacA조합기인형이sla/m2/il위주(62.2%,74/119),sla/mlb/il차지(25.2%,30/119).불동지구、불동질병래원균주sla분포적차이무통계학의의(P>005).이m구기인다태성재질병류형급분리지구간차이유통계학의의(P<0.01).불동질병래원균주간i구기인분형분포적차이무통계학의의,단불동분리지구균주간차이유통계학의의(P<0.01).119주적vacA기인서렬취류위삼개불동조군.결론 HP중국분리주vacA기인형이sla/m2/il조합형위주,sla분형결과여균주분리지구급질병류형무관.불동지구、불동질병래원균주m구기인분형불동.불동지구균주vacA기인i구분형불동,단i구기인분형여균주적질병래원무현저상관성.
Objective To understand the polymorphism of Helicobacter pylori (H. pylori) vacA alleles in China. Methods A total of 119 H. pylori strains were isolated from different gastroduodenal diseases in 7 different geographic regions in China. vacA and its alleles were identified according to the length of PCR products with DNA electrophoresis. The distributions of vacA alleles were statistically analyzed. The core fragment of vacA was sequentially analyzed by software MEGA4.0. Results The alleles in vacA dominantly belonged to sla, m2 and il in the tested strains.The distribution appeared to be 97.5%(116/119) ,68.9%(82/119) and 91.6%(109/119),respectively.The mlb allele appeared to be 26.1% (31/119). slb and mla were not found. The major vacA recombination was between slaim2/il and 62.2% , followed by sla/mlb/il (25.2% , 30/119). No association was found between the distribution of sla allele and the clinical outcome, as well as the geographical regions (P>0.05). However, the distribution of m alleles showed significant difference both among the types of disease and the geographic regions (P<0.01), The present of i alleles did not show significant differences among disease patterns, but had significant differences between different geographic groups (P<0.01). Three clusters were identified among these 119 isolates according to the DNA sequence of vacA. Conclusion sla/m2/il appeared to be the main allele in H. pylori vacA isolates from China in this study. The distribution of m alleles in vacA was correlated both to the regions and the disease patterns. The presence of i allele was associated to the regions but not the disease patterns.
