CAJ | 학술논문

目的探讨轮状病毒肠道外感染患儿血清甘露聚糖结合蛋白(MBP)水平的变化及其与轮状病毒肠道外感染的关系.方法采用双抗体夹心酶联免疫吸附法(ELISA)测定76例轮状病毒肠道外感染患儿和63例单纯轮状病毒肠炎患儿不同病程中的血清MBP水平以及50例健康对照组小儿血清MBP水平.结果轮状病毒肠道外感染患儿急性期血清MBP为(176.35±113.12)μg/L,明显低于单纯轮状病毒肠炎急性期水平(392.27±128.96)μg/L以及健康对照组小儿MBP血清水平(676.25±248.63)μg/L,差异有显著性(P＜0.001);轮状病毒肠道外感染患儿恢复期血清MBP水平为(358.63±106.54)μg/L,低于单纯轮状病毒肠炎恢复期水平[(558.49±173.24)μg/L]以及健康对照组小儿血清MBP水平,差异有显著性(P＜0.001);轮状病毒肠道外感染导致的肺炎、肝损害、心肌损害以及中枢神经系统损害急性期患儿血清MBP水平分别为(198.24±126.47)μg/L、(169.34±124.38)μg/L、(184.62±123.64)μg/L、(180.74±126.86)μg/L,差异无显著性(P＞0.05).结论轮状病毒肠道外感染患儿急性期及恢复期血清MBP水平明显低于单纯轮状病毒肠炎急性期及恢复期血清MBP水平,但轮状病毒肠道外感染导致的不同肠道外脏器损害患儿急性期血清MBP水平无显著差异;轮状病毒肠道外感染的发生与血清MBP水平低下密切相关.
목적 탐토륜상병독장도외감염환인혈청감로취당결합단백(MBP)수평적변화급기여륜상병독장도외감염적관계.방법 채용쌍항체협심매련면역흡부법(ELISA)측정76례륜상병독장도외감염환인화63례단순륜상병독장염환인불동병정중적혈청MBP수평이급50례건강대조조소인혈청MBP수평.결과 륜상병독장도외감염환인급성기혈청MBP위(176.35±113.12)μg/L,명현저우단순륜상병독장염급성기수평(392.27±128.96)μg/L이급건강대조조소인MBP혈청수평(676.25±248.63)μg/L,차이유현저성(P＜0.001);륜상병독장도외감염환인회복기혈청MBP수평위(358.63±106.54)μg/L,저우단순륜상병독장염회복기수평[(558.49±173.24)μg/L]이급건강대조조소인혈청MBP수평,차이유현저성(P＜0.001);륜상병독장도외감염도치적폐염、간손해、심기손해이급중추신경계통손해급성기환인혈청MBP수평분별위(198.24±126.47)μg/L、(169.34±124.38)μg/L、(184.62±123.64)μg/L、(180.74±126.86)μg/L,차이무현저성(P＞0.05).결론 륜상병독장도외감염환인급성기급회복기혈청MBP수평명현저우단순륜상병독장염급성기급회복기혈청MBP수평,단륜상병독장도외감염도치적불동장도외장기손해환인급성기혈청MBP수평무현저차이;륜상병독장도외감염적발생여혈청MBP수평저하밀절상관.
Objective To explore the relationship between extraintestinal rotavirus infections and serum MBP levels. Methods Serum MBP levels were measured by double-antibody sandwich enzyme-linked immunosorbent assays (ELISA) in children including extraintestinal rotavirus infections (n = 76) and common rotavirus enteritis ( n = 63 ) during the acute and convalescence phases. A group of healthy children ( n =50) were recruited as control. Results MBP levels were significantly lower in patients with extraintestinal rotavirus infections( 176.35 ± 113.12 ) μg/L in acute phases than those in patients with common rotavirus enteritis (392. 27 ± 128.96) μg/L and healthy control group(676. 25 ± 248. 63) μg/L, and the difference was significant (P ＜0. 001 ). The serum MBP levels in convalescence phases in the group of extraintestinal rotavirus infections( 358.63 ± 106. 54 ) μg/L was lower than those in the group of common rotavirus enteritis (558. 49 ± 173. 24 ) μg/L and the healthy controls, and their difference was significant ( P ＜ 0. 001 ). The MBP levels in the acute phases among pneumonia group, hepatic lesion group, cardiac damage group and central nervous system damage group caused by rotavirus infection were ( 198.24 ± 126.47) μg/L, ( 169.34 ±124. 38) μg/L,( 184. 62 ± 123.64) μg/L, ( 180. 74 ± 126. 86) μg/L, respectively. The difference among those groups was not significant ( P ＞ 0. 05 ). Conclusion Patients with extraintestinal rotavirus infections showed significantly lower MBP levels during acute and convalescence phases than patients with common rotavirus enteritis. But MBP levels showed no significant differences among those groups of patients with different extraintestinal organ damage caused by rotavirus infection. Lower MBP levels may be associated with the increased susceptibility to extraintestinal rotavirus infections.