中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
3期
332-335
,共4页
霍奇金病/并发症/代谢%爱泼斯坦巴尔病毒感染/并发症/代谢%基因,p16
霍奇金病/併髮癥/代謝%愛潑斯坦巴爾病毒感染/併髮癥/代謝%基因,p16
곽기금병/병발증/대사%애발사탄파이병독감염/병발증/대사%기인,p16
Hodgkin disease/CO/ME%Epstein-barr virus infections/CO/ME%Genes,p16
目的 探讨EB病毒在经典型霍奇金淋巴瘤(CHL)中的感染和p16蛋白表达的关系.方法 应用EBER-1寡核苷酸探针以原位杂交方法检测80例CHL组织中的EBV编码的小RNA;用免疫组化方法检测p16蛋白表达.结果 80例CHL患者中,男52例,女28例,男女之比为1.86:1,其中52例CHL男性患者中,EBER-1阳性率63.46%;28例CHL女性患者中,EBER-1阳性率39.29%,两者比较具有统计学意义(x2=4.298,P=0.038).儿童和老年人中CHL患者共45例,其EBV表达阳性率75.56%,多于成年人的28.57%,两者比较差异具有统计学意义(x2=20.13,P=0.000).EBER-1阳性表达定位在R-S细胞核上,其阳性表达率为55.00%,各亚型CHL的EBV潜伏感染检出率分别是:混合细胞型( MC)71.79%,淋巴消减型(LD) 42.85%,淋巴细胞为主型(LP)47.06%,结节硬化型( NS)29.41%,且MC型明显高于NS型(x2=8.787,P=0.003);p16蛋白阳性表达定位在R-S细胞的细胞核和细胞浆,其总的阳性表达率为45%,各亚型阳性率分别为:LP 47.05%,MC 48.71%,NS35.29%,LD 42.85%;p16蛋白与EBER-1之间呈负相关(r=-0.242,P<0.05).结论 CHL发生可能与EBV感染有关,且p16蛋白的高表达为协助诊断CHL提供了依据.
目的 探討EB病毒在經典型霍奇金淋巴瘤(CHL)中的感染和p16蛋白錶達的關繫.方法 應用EBER-1寡覈苷痠探針以原位雜交方法檢測80例CHL組織中的EBV編碼的小RNA;用免疫組化方法檢測p16蛋白錶達.結果 80例CHL患者中,男52例,女28例,男女之比為1.86:1,其中52例CHL男性患者中,EBER-1暘性率63.46%;28例CHL女性患者中,EBER-1暘性率39.29%,兩者比較具有統計學意義(x2=4.298,P=0.038).兒童和老年人中CHL患者共45例,其EBV錶達暘性率75.56%,多于成年人的28.57%,兩者比較差異具有統計學意義(x2=20.13,P=0.000).EBER-1暘性錶達定位在R-S細胞覈上,其暘性錶達率為55.00%,各亞型CHL的EBV潛伏感染檢齣率分彆是:混閤細胞型( MC)71.79%,淋巴消減型(LD) 42.85%,淋巴細胞為主型(LP)47.06%,結節硬化型( NS)29.41%,且MC型明顯高于NS型(x2=8.787,P=0.003);p16蛋白暘性錶達定位在R-S細胞的細胞覈和細胞漿,其總的暘性錶達率為45%,各亞型暘性率分彆為:LP 47.05%,MC 48.71%,NS35.29%,LD 42.85%;p16蛋白與EBER-1之間呈負相關(r=-0.242,P<0.05).結論 CHL髮生可能與EBV感染有關,且p16蛋白的高錶達為協助診斷CHL提供瞭依據.
목적 탐토EB병독재경전형곽기금림파류(CHL)중적감염화p16단백표체적관계.방법 응용EBER-1과핵감산탐침이원위잡교방법검측80례CHL조직중적EBV편마적소RNA;용면역조화방법검측p16단백표체.결과 80례CHL환자중,남52례,녀28례,남녀지비위1.86:1,기중52례CHL남성환자중,EBER-1양성솔63.46%;28례CHL녀성환자중,EBER-1양성솔39.29%,량자비교구유통계학의의(x2=4.298,P=0.038).인동화노년인중CHL환자공45례,기EBV표체양성솔75.56%,다우성년인적28.57%,량자비교차이구유통계학의의(x2=20.13,P=0.000).EBER-1양성표체정위재R-S세포핵상,기양성표체솔위55.00%,각아형CHL적EBV잠복감염검출솔분별시:혼합세포형( MC)71.79%,림파소감형(LD) 42.85%,림파세포위주형(LP)47.06%,결절경화형( NS)29.41%,차MC형명현고우NS형(x2=8.787,P=0.003);p16단백양성표체정위재R-S세포적세포핵화세포장,기총적양성표체솔위45%,각아형양성솔분별위:LP 47.05%,MC 48.71%,NS35.29%,LD 42.85%;p16단백여EBER-1지간정부상관(r=-0.242,P<0.05).결론 CHL발생가능여EBV감염유관,차p16단백적고표체위협조진단CHL제공료의거.
Objective To study the relationship between EBV infection of classic Hodgkin's lyphoma and expression of p16 protein.Methods EBER-1 and p16 protein expression in 80 cases of CHL were studied with EBER-1 oligonucleotide probe and immunohistochemistry respectively.Results 80 cases of CHL were enrolled in this study,including male 52 cases,28 cases of female,male to female ratio of 1.86 ∶ 1.The positive rate of EBER-1 in female was 39.29% and in male was 63.46%,which have statistically significant difference (x2 =4.298,P =0.038).The positive rate of EBER-1 in children and the older was significantly higher than that in adult (x2 =20.13,P =0.000).EBER-1 positive expression located in the R-S nuclei which positive expression rate was 55.00%,including mixed cell type (MC) 71.79%,lymphocyte depletion type (LD) 42.85%.lymphocyte predominance (LP) 47.06% and nodular sclerosis type (NS) 29.41%.MG type was significantly higher than NS ( x2 =8.787,P =0.003 ) ; p16 was noted in thc nucleus and cytoplasm.The total positive expression rate was 45%,and subtype positive rates were 47.05% (LP),48.71% ( MC),35.29% (NS) and 42.85% (LD).There was a negative correlation between p16 protein and EBER-1 ( r =-0.242,P < 0.05 ).Conclusions CHL may be related to EBV latent infection and it can be considered as potential markers to identify HRS cells in diagnosis.