中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
3期
332-335
,共4页
霍奇金病/并发症/代谢%爱泼斯坦巴尔病毒感染/并发症/代谢%基因,p16
霍奇金病/併髮癥/代謝%愛潑斯坦巴爾病毒感染/併髮癥/代謝%基因,p16
곽기금병/병발증/대사%애발사탄파이병독감염/병발증/대사%기인,p16
Hodgkin disease/CO/ME%Epstein-barr virus infections/CO/ME%Genes,p16
目的 探讨EB病毒在经典型霍奇金淋巴瘤(CHL)中的感染和p16蛋白表达的关系.方法 应用EBER-1寡核苷酸探针以原位杂交方法检测80例CHL组织中的EBV编码的小RNA；用免疫组化方法检测p16蛋白表达.结果 80例CHL患者中,男52例,女28例,男女之比为1.86:1,其中52例CHL男性患者中,EBER-1阳性率63.46％；28例CHL女性患者中,EBER-1阳性率39.29％,两者比较具有统计学意义(x2=4.298,P=0.038).儿童和老年人中CHL患者共45例,其EBV表达阳性率75.56％,多于成年人的28.57％,两者比较差异具有统计学意义(x2=20.13,P=0.000).EBER-1阳性表达定位在R-S细胞核上,其阳性表达率为55.00％,各亚型CHL的EBV潜伏感染检出率分别是:混合细胞型( MC)71.79％,淋巴消减型(LD) 42.85％,淋巴细胞为主型(LP)47.06％,结节硬化型( NS)29.41％,且MC型明显高于NS型(x2=8.787,P=0.003)；p16蛋白阳性表达定位在R-S细胞的细胞核和细胞浆,其总的阳性表达率为45％,各亚型阳性率分别为:LP 47.05％,MC 48.71％,NS35.29％,LD 42.85％；p16蛋白与EBER-1之间呈负相关(r=-0.242,P＜0.05).结论 CHL发生可能与EBV感染有关,且p16蛋白的高表达为协助诊断CHL提供了依据.
目的 探討EB病毒在經典型霍奇金淋巴瘤(CHL)中的感染和p16蛋白錶達的關繫.方法 應用EBER-1寡覈苷痠探針以原位雜交方法檢測80例CHL組織中的EBV編碼的小RNA；用免疫組化方法檢測p16蛋白錶達.結果 80例CHL患者中,男52例,女28例,男女之比為1.86:1,其中52例CHL男性患者中,EBER-1暘性率63.46％；28例CHL女性患者中,EBER-1暘性率39.29％,兩者比較具有統計學意義(x2=4.298,P=0.038).兒童和老年人中CHL患者共45例,其EBV錶達暘性率75.56％,多于成年人的28.57％,兩者比較差異具有統計學意義(x2=20.13,P=0.000).EBER-1暘性錶達定位在R-S細胞覈上,其暘性錶達率為55.00％,各亞型CHL的EBV潛伏感染檢齣率分彆是:混閤細胞型( MC)71.79％,淋巴消減型(LD) 42.85％,淋巴細胞為主型(LP)47.06％,結節硬化型( NS)29.41％,且MC型明顯高于NS型(x2=8.787,P=0.003)；p16蛋白暘性錶達定位在R-S細胞的細胞覈和細胞漿,其總的暘性錶達率為45％,各亞型暘性率分彆為:LP 47.05％,MC 48.71％,NS35.29％,LD 42.85％；p16蛋白與EBER-1之間呈負相關(r=-0.242,P＜0.05).結論 CHL髮生可能與EBV感染有關,且p16蛋白的高錶達為協助診斷CHL提供瞭依據.
목적 탐토EB병독재경전형곽기금림파류(CHL)중적감염화p16단백표체적관계.방법 응용EBER-1과핵감산탐침이원위잡교방법검측80례CHL조직중적EBV편마적소RNA；용면역조화방법검측p16단백표체.결과 80례CHL환자중,남52례,녀28례,남녀지비위1.86:1,기중52례CHL남성환자중,EBER-1양성솔63.46％；28례CHL녀성환자중,EBER-1양성솔39.29％,량자비교구유통계학의의(x2=4.298,P=0.038).인동화노년인중CHL환자공45례,기EBV표체양성솔75.56％,다우성년인적28.57％,량자비교차이구유통계학의의(x2=20.13,P=0.000).EBER-1양성표체정위재R-S세포핵상,기양성표체솔위55.00％,각아형CHL적EBV잠복감염검출솔분별시:혼합세포형( MC)71.79％,림파소감형(LD) 42.85％,림파세포위주형(LP)47.06％,결절경화형( NS)29.41％,차MC형명현고우NS형(x2=8.787,P=0.003)；p16단백양성표체정위재R-S세포적세포핵화세포장,기총적양성표체솔위45％,각아형양성솔분별위:LP 47.05％,MC 48.71％,NS35.29％,LD 42.85％；p16단백여EBER-1지간정부상관(r=-0.242,P＜0.05).결론 CHL발생가능여EBV감염유관,차p16단백적고표체위협조진단CHL제공료의거.
Objective To study the relationship between EBV infection of classic Hodgkin's lyphoma and expression of p16 protein.Methods EBER-1 and p16 protein expression in 80 cases of CHL were studied with EBER-1 oligonucleotide probe and immunohistochemistry respectively.Results 80 cases of CHL were enrolled in this study,including male 52 cases,28 cases of female,male to female ratio of 1.86 ∶ 1.The positive rate of EBER-1 in female was 39.29％ and in male was 63.46％,which have statistically significant difference (x2 =4.298,P =0.038).The positive rate of EBER-1 in children and the older was significantly higher than that in adult (x2 =20.13,P =0.000).EBER-1 positive expression located in the R-S nuclei which positive expression rate was 55.00％,including mixed cell type (MC) 71.79％,lymphocyte depletion type (LD) 42.85％.lymphocyte predominance (LP) 47.06％ and nodular sclerosis type (NS) 29.41％.MG type was significantly higher than NS ( x2 =8.787,P =0.003 ) ; p16 was noted in thc nucleus and cytoplasm.The total positive expression rate was 45％,and subtype positive rates were 47.05％ (LP),48.71％ ( MC),35.29％ (NS) and 42.85％ (LD).There was a negative correlation between p16 protein and EBER-1 ( r =-0.242,P ＜ 0.05 ).Conclusions CHL may be related to EBV latent infection and it can be considered as potential markers to identify HRS cells in diagnosis.
