中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
1期
34-37
,共4页
刘蔚然%徐诚实%吴安石%岳云
劉蔚然%徐誠實%吳安石%嶽雲
류위연%서성실%오안석%악운
异氟醚%淀粉样β蛋白%认知障碍%老年人%海马
異氟醚%澱粉樣β蛋白%認知障礙%老年人%海馬
이불미%정분양β단백%인지장애%노년인%해마
Isoflurane%Amyloid beta-protein%Cognition disorders%Aged%Hippocampus
目的 探讨异氟醚麻醉对老龄大鼠海马淀粉样β蛋白(Aβ)表达的影响.方法 健康雄性SD大鼠61只,18～ 19月龄,体重400～500 g,采用随机数字表法,将其随机分为3组,异氟醚组(Ⅰ组,n=31):1.4％异氟醚麻醉2h,于麻醉前5d和麻醉后2d分别行Morris水迷宫实验以评价认知功能；对照组(Ⅱ组,n=20):仅行Morris水迷宫实验；空白组(Ⅲ组,n=10):不行异氟醚麻醉和Morris水迷宫实验.根据是否发生认知功能障碍将Ⅰ组分为2亚组:认知功能障碍组(P亚组)和非认知功能障碍组(NP亚组).行为学测试结束后,处死大鼠,分离海马,测定海马Aβ40、Aβ42、β内分泌酶(BACE)、胰岛素降解酶(IDE)和中性内肽酶(NEP)的水平.结果 三组大鼠麻醉前Morris水迷宫实验结果比较差异无统计学意义(P＞0.05)；Ⅰ组和Ⅱ组游泳速度比较差异无统计学意义(P＞0.05),P亚组平台所在原象限停留时间短于NP亚组和Ⅱ组(P＜0.01),NP亚组和Ⅱ组平台所在原象限停留时间比较差异无统计学意义(P＞0.05)；三组大鼠海马Aβ40、Aβ42、BACE、NEP和IDE水平比较差异无统计学意义,P亚组与NP亚组比较差异无统计学意义(P＞0.05).结论 异氟醚麻醉对老龄大鼠海马Aβ表达无影响,提示异氟醚诱导的老龄大鼠认知功能障碍与Aβ通路无关.
目的 探討異氟醚痳醉對老齡大鼠海馬澱粉樣β蛋白(Aβ)錶達的影響.方法 健康雄性SD大鼠61隻,18～ 19月齡,體重400～500 g,採用隨機數字錶法,將其隨機分為3組,異氟醚組(Ⅰ組,n=31):1.4％異氟醚痳醉2h,于痳醉前5d和痳醉後2d分彆行Morris水迷宮實驗以評價認知功能；對照組(Ⅱ組,n=20):僅行Morris水迷宮實驗；空白組(Ⅲ組,n=10):不行異氟醚痳醉和Morris水迷宮實驗.根據是否髮生認知功能障礙將Ⅰ組分為2亞組:認知功能障礙組(P亞組)和非認知功能障礙組(NP亞組).行為學測試結束後,處死大鼠,分離海馬,測定海馬Aβ40、Aβ42、β內分泌酶(BACE)、胰島素降解酶(IDE)和中性內肽酶(NEP)的水平.結果 三組大鼠痳醉前Morris水迷宮實驗結果比較差異無統計學意義(P＞0.05)；Ⅰ組和Ⅱ組遊泳速度比較差異無統計學意義(P＞0.05),P亞組平檯所在原象限停留時間短于NP亞組和Ⅱ組(P＜0.01),NP亞組和Ⅱ組平檯所在原象限停留時間比較差異無統計學意義(P＞0.05)；三組大鼠海馬Aβ40、Aβ42、BACE、NEP和IDE水平比較差異無統計學意義,P亞組與NP亞組比較差異無統計學意義(P＞0.05).結論 異氟醚痳醉對老齡大鼠海馬Aβ錶達無影響,提示異氟醚誘導的老齡大鼠認知功能障礙與Aβ通路無關.
목적 탐토이불미마취대노령대서해마정분양β단백(Aβ)표체적영향.방법 건강웅성SD대서61지,18～ 19월령,체중400～500 g,채용수궤수자표법,장기수궤분위3조,이불미조(Ⅰ조,n=31):1.4％이불미마취2h,우마취전5d화마취후2d분별행Morris수미궁실험이평개인지공능；대조조(Ⅱ조,n=20):부행Morris수미궁실험；공백조(Ⅲ조,n=10):불행이불미마취화Morris수미궁실험.근거시부발생인지공능장애장Ⅰ조분위2아조:인지공능장애조(P아조)화비인지공능장애조(NP아조).행위학측시결속후,처사대서,분리해마,측정해마Aβ40、Aβ42、β내분비매(BACE)、이도소강해매(IDE)화중성내태매(NEP)적수평.결과 삼조대서마취전Morris수미궁실험결과비교차이무통계학의의(P＞0.05)；Ⅰ조화Ⅱ조유영속도비교차이무통계학의의(P＞0.05),P아조평태소재원상한정류시간단우NP아조화Ⅱ조(P＜0.01),NP아조화Ⅱ조평태소재원상한정류시간비교차이무통계학의의(P＞0.05)；삼조대서해마Aβ40、Aβ42、BACE、NEP화IDE수평비교차이무통계학의의,P아조여NP아조비교차이무통계학의의(P＞0.05).결론 이불미마취대노령대서해마Aβ표체무영향,제시이불미유도적노령대서인지공능장애여Aβ통로무관.
Objective To investigate the effect of isoflurane anesthesia on the expression of amyloid β protein (Aβ) in hippocampus in aged rats.Methods Sixty-one healthy male SD rats,aged 18-19 months,weighing 400-500 g,were randomly divided into 3 groups:isoflurane group (group Ⅰ,n =31),control group (groupⅡ,n =20),and blank group (group Ⅲ,n =10).Group Ⅰ inhaled 1.4％ isoflurane for 2 h,and cognitive function was assessed at 5 d before and 2 d after anesthesia by Morris water maze.Group Ⅱ received Morris water maze text only.Group Ⅲ received no treatment.Group Ⅰ was divided into 2 subgroups according to whether the cognitive impairment occurred:cognitive impairment group (P subgroup) and no cognitive impairment group (NP subgroup).The animals were sacrificed immediately after the test.The hippocampus was isolated for determination of the levels of Aβ40 and Aβ42,β-secretase (BACE),insulin degrading enzyme (IDE)and neprilysin (NEP).Results There was no significant difference in the results of Morris water maze test before anesthesia among the 3 groups.There was no significant difference in the swimming velocity between group Ⅰ and group Ⅱ.The time of staying at the original platform quadrant was significantly shorter in P subgroup than in NP subgroup and Ⅱ group,and there was no significant difference in the time of staying at the original platform quadrant between NP subgroup and Ⅱ group.There was no significant difference in the levels of Aβ40 and Aβ42,BACE,NEP and IDE among the 5 groups,and between P subgroup and NP subgroup.Conclusion Isoflurane anesthesia has no effect on the expression of Aβ in the hippocampus,indicating that isoflurane-induced cognitive impairment is not related to Aβ pathway in aged rats.
