CAJ | 학술논문

目的探索特异性siRNA靶向干扰恶性胶质瘤U251细胞中INTα_vβ_3表达后对细胞生长的作用.方法将INTα_vβ_3特异性siRNA经脂质体(LipofectamineTM~(2000))转染U251细胞.RT-PCR、免疫细胞化学和Western blot法检测INTα_vβ_3蛋白表达.MTT检测干扰后细胞增殖变化.结果 INTα_vβ_3特异siRNA对U251细胞增殖有明显抑制作用.siRNA组、脂质体组与对照组各时间点的结果差异有统计学意义.免疫细胞化学对照组和脂质体组均见INTα_vβ_3呈绿色表达,siRNA组表达降低.Western blot法和RT-PCR结果证实转染特异siRNA后的细胞在蛋白及mRNA水平均能抑制INTα_vβ_3的表达.结论特异性siRNA可干扰INTα_vβ_3在脑胶质瘤U251细胞中的表达并抑制细胞增殖.
목적 탐색특이성siRNA파향간우악성효질류U251세포중INTα_vβ_3표체후대세포생장적작용.방법 장INTα_vβ_3특이성siRNA경지질체(LipofectamineTM~(2000))전염U251세포.RT-PCR、면역세포화학화Western blot법검측INTα_vβ_3단백표체.MTT검측간우후세포증식변화.결과 INTα_vβ_3특이siRNA대U251세포증식유명현억제작용.siRNA조、지질체조여대조조각시간점적결과 차이유통계학의의.면역세포화학대조조화지질체조균견INTα_vβ_3정록색표체,siRNA조표체강저.Western blot법화RT-PCR결과 증실전염특이siRNA후적세포재단백급mRNA수평균능억제INTα_vβ_3적표체.결론 특이성siRNA가간우INTα_vβ_3재뇌효질류U251세포중적표체병억제세포증식.
Objective Explore the specificity of siRNA interference targeting malignant glioma U251 cells, INTα_vβ_3 expression of cell growth after the role. Method U251 cells were transfected with INT α_vβ_3 specific siRNA by lipofectamine(LipefectamineTM~(2000)). RT-PCR, immune ceils staining and Western blot were used to determine the expression of INTα_vβ_3. Cell proliferation after the detection of changes in interference was tested by MTT. Results INTα_vβ_3 specific siRNA shown to inhibit U251 cell proliferation. There were statistical significance between siRNA group, liposome group and the control group at different time points. Green INTα_vβ_3 expression was found in control group and lipesome groups, and reduced in siRNA group. Western blotting and RT-PCR results shown that the expression of integrin could be inhibited by α_vβ_3 transfection in the protein and mRNA level Conclusions Integrin-specific siRNA INTα_vβ_3 can inhibit the U251 glioma cell proliferation.