CAJ | 학술논문

目的通过乙型肝炎流行病学调查和病毒基因检测,了解乙肝疫苗长期免疫地区低年龄人群的基因序列与突变特征.方法从乙肝监测点收集乙型肝炎病毒表面抗原阳性血清,取其中年龄小于16岁者,扩增包括preS和S基因在内的基因序列片段,共1100碱基,序列测定后与标准基因型别比较,确定病毒基因及血清型别,确定a抗原决定簇的氨基酸替代突变发生率,选取一株病毒进行全基因扩增、克隆和序列测定.结果共检测样本35例,33例血清扩增出乙肝病毒基因序列,其中30例乙型肝炎病毒基因型为B型,占90.9%,3例乙型肝炎病毒基因型为C型,占9.0%,1例血清型为ayw,3例为adr,其余29例为adw,共有5例在a抗原决定簇发生氨基酸替代突变,发生率约为15.2%.其中5856号血清扩增乙型肝炎病毒全基因为B型,血清型为adw,共3215个碱基.结论该地区乙型肝炎基因型主要为B型,血清型为adw,人群中a抗原决定簇某些位点已发生与疫苗免疫逃逸相关的突变.
목적 통과을형간염류행병학조사화병독기인검측,료해을간역묘장기면역지구저년령인군적기인서렬여돌변특정.방법 종을간감측점수집을형간염병독표면항원양성혈청,취기중년령소우16세자,확증포괄preS화S기인재내적기인서렬편단,공1100감기,서렬측정후여표준기인형별비교,학정병독기인급혈청형별,학정a항원결정족적안기산체대돌변발생솔,선취일주병독진행전기인확증、극륭화서렬측정.결과 공검측양본35례,33례혈청확증출을간병독기인서렬,기중30례을형간염병독기인형위B형,점90.9%,3례을형간염병독기인형위C형,점9.0%,1례혈청형위ayw,3례위adr,기여29례위adw,공유5례재a항원결정족발생안기산체대돌변,발생솔약위15.2%.기중5856호혈청확증을형간염병독전기인위B형,혈청형위adw,공3215개감기.결론 해지구을형간염기인형주요위B형,혈청형위adw,인군중a항원결정족모사위점이발생여역묘면역도일상관적돌변.
Objective In order to investigate the characterization of mutation and genotype distributing in the younger group which was under the universal vaccination. The sequence of HBV was analyzed to offer the information to control and prevention in the area. Methods young person's sera with positive HBsAg are collected, and the Large S sequence of HBV including preS and S gene are amplified and sequenced. The genotype and serotype were determined by clastwal with the standard genotype sequence. And one virus complete genome is amplified. Results The virus gene are successful amplified from the 33 sera. The sequence result indicate the 30of 33 (90.9%) HBV genotype is B and 3 of 33 (9.0%) is C. The HBV serotype including ayw (1), adr (3), adw (29), 5 of 33 mutated in the "a" dominant of HBV, and the percentage is 15.2%. The HBV full length gene of serum number of 5856 is amplified and sequenced. Its genotype is B, serotype is adw and length is 3215 base. Conclusion s The dominant genotype of HuNan is B, and t he dominant serotype is adw.