CAJ | 학술논문

目的研制聚乙醇酸聚乳酸共聚物(PLGA)材料的纵向取向支架,观察体外生物相容性、细胞在支架内分布等特性,探讨其作为关节软骨组织工程支架的可能性.方法相分离法制备纵向取向PLGA支架,等离子改性、胶原包埋处理,体外接种关节软骨细胞,在环境扫描电镜下观察细胞生长、分布情况.结果研制的PLGA取向支架生物相容性明显提高,细胞沿纵向管道垂直排列,细胞分布均匀,单侧加载细胞进入深度可达2.5 nlln.结论取向支架能明显改善细胞在载体内的分布,细胞纵向排列接近关节软骨细胞排列方式.
목적 연제취을순산취유산공취물(PLGA)재료적종향취향지가,관찰체외생물상용성、세포재지가내분포등특성,탐토기작위관절연골조직공정지가적가능성.방법 상분리법제비종향취향PLGA지가,등리자개성、효원포매처리,체외접충관절연골세포,재배경소묘전경하관찰세포생장、분포정황.결과 연제적PLGA취향지가생물상용성명현제고,세포연종향관도수직배렬,세포분포균균,단측가재세포진입심도가체2.5 nlln.결론 취향지가능명현개선세포재재체내적분포,세포종향배렬접근관절연골세포배렬방식.
Objective To explore the method of fabricating oriental scaffolds and investigate the biocompatibility of the scaffolds as well as cells distribution within the scaffolds in vitro. Methods The oriental poly (lactic-co-glycolic acid) (PLGA) scaffolds were fabricated with modified emulsion-phase separation method. The scaffolds were treated with plasma and then anchored with collagen I. Artiticular chondrocytes were loaded into the scaffolds. The growth status and distributing characteristic of the cells were investigated by environmental scanning electron microscope. Results The scaffold was well compatible with the articular chondrocytes. The cells could reach to 2. 5 mm depth with unilateral loading. The cells distributed evenly in the scaffold and lined along the inner pipes. Conclusions The oriental scaffold fabricated could significantly promote the distributing characteristics of the chondrocytes. The vertical alignment of the chondrocytes within the scaffold is closely simular to that of artilage cartilage.