CAJ | 학술논문

背景:人类载脂蛋白H具有多态性,其与三酰甘油代谢及血小板功能有关,因而推测与冠状动脉粥样硬化性心脏病(冠心病)可能有一定的关系.目的:分析载脂蛋白H外显子8基因多态性与冠心病及脂代谢的相关性.设计:病例-对照观察.单位:山东省青岛市海慈医疗集团心内科.对象:选择青岛市海慈医疗集团心内科收治110例冠心病患者(经冠状动脉造影证实)为冠心病组,同期体检的100例健康志愿者为对照组.方法:抽取所有受试者外周静脉血2 mL,采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了2组的载脂蛋白H外显子8基因型;同时采用氧化酶法测定血脂.主要观察指标:①载脂蛋白H外显子8基因型多态性分析结果.②两组载脂蛋白H外显子(Try316Ser)基因频率.③两组不同基因型者的血脂水平比较. 结果:210例全部进入结果分析.①载脂蛋白H基因8号外显子存在G1025C(Try316Ser)多态,分别为GG和GC基因型,未发现CC型.②冠心病组GC基因型的频率为25.5%,C等位基因频率为0.13,显著高于对照组(10.0%,0.05,P＜0.05).③冠心病组GC基因型者的三酰甘油水平显著高于GG型和及对照组的各基因型[(1.38±0.24),(1.16±0.10),(1.09±0.78),(1.12±0.76)mmol/L,P＜0.05];冠心病组低密度脂蛋白胆固醇高于对照组(P＜0.05).结论:载脂蛋白H外显子8基因多态性与冠心病、三酰甘油相关.
배경:인류재지단백H구유다태성,기여삼선감유대사급혈소판공능유관,인이추측여관상동맥죽양경화성심장병(관심병)가능유일정적관계.목적:분석재지단백H외현자8기인다태성여관심병급지대사적상관성.설계:병례-대조관찰.단위:산동성청도시해자의료집단심내과.대상:선택청도시해자의료집단심내과수치110례관심병환자(경관상동맥조영증실)위관심병조,동기체검적100례건강지원자위대조조.방법:추취소유수시자외주정맥혈2 mL,채용취합매련식반응결합한제성편단장도다태분석방법,분석료2조적재지단백H외현자8기인형;동시채용양화매법측정혈지.주요관찰지표:①재지단백H외현자8기인형다태성분석결과.②량조재지단백H외현자(Try316Ser)기인빈솔.③량조불동기인형자적혈지수평비교. 결과:210례전부진입결과분석.①재지단백H기인8호외현자존재G1025C(Try316Ser)다태,분별위GG화GC기인형,미발현CC형.②관심병조GC기인형적빈솔위25.5%,C등위기인빈솔위0.13,현저고우대조조(10.0%,0.05,P＜0.05).③관심병조GC기인형자적삼선감유수평현저고우GG형화급대조조적각기인형[(1.38±0.24),(1.16±0.10),(1.09±0.78),(1.12±0.76)mmol/L,P＜0.05];관심병조저밀도지단백담고순고우대조조(P＜0.05).결론:재지단백H외현자8기인다태성여관심병、삼선감유상관.
BACKGROUND: Human apolipoprotein H which characterizes by polymorphism is related to metabolism of triacylglycerol (TG) and function of platelet; therefore, it is suspected that it is possibly related to coronary atherosclerotic heart disease (CAHD).OBJECTIVE: To investigate the correlation among apolipoprotein H(ApoH) exon-8 polymorphism with CAHD and its effect on lipometabolism.DESIGN: Case-controlled observation.SETTING: Department of Cardiology, Haici Medical Group, Qingdao,Shangdong. PARTICIPANTS: 110 CAHD patients selected from Qingdao Haici Medical Group were regarded as CAHD group, and other 100 healthy subjects were regarded as control group.METHODS: 2 mL blood was collected from peripheral vein of all cases. Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotype of ApoH exon-8; meanwhile, lipids were measured with oxidase technique.MAIN OUTCOME MEASURES: ① polymorphism of ApoH exon-8 genotype; ② gene frequency of ApoH exon-8 (Try316Ser); ③ comparisons of lipid level of cases with various genetypes.RESULTS: All 210 cases were involved in the final analysis. ① G1025C (Try316Ser) existed in ApoH exon-8, including types of GG and GC, butnot CC type. ② The frequency of GC in the CAHD group was 25.5％, and the frequency of C allele was 0.13; they were significantly higher than those in control group (10％, 0.05, P ＜0.05). ③ TG level in genotype GC of CAHD group was significantly higher than that both in GG genotype and in any genotype of control group [(1.38±0.24), (1.16±0.10),(1.09±0.78), (1.12±0.76) mmol/L, P ＜ 0.05]. Level of low-density lipoprotein cholesterol (C-LDL) was higher in CAHD group than that in control group (P＜0.05).CONCLUSION: The polymorphism of ApoH exon-8 is closely related to CAHD and TG level.