CAJ | 학술논문

背景:透明质酸关节腔注射治疗骨关节炎已经成为一种常规方法,但对大骨节病的治疗还正在推广,施加透明质酸后对大骨节病患者软骨细胞代谢的影响,目前尚为空白.目的:课题创新性设计观察透明质酸对体外培养的大骨节病软骨细胞分泌Ⅱ型胶原和聚集蛋白聚糖的影响,以期能指导临床治疗.设计、时间及地点:对比观察,实验于2006-09/2009-01在西安交通大学医学院生物医学研究实验中心和地方病实验室完成.材料:大骨节关节病标本来源于6例大骨节关节病患者实施膝关节手术摘除的软骨及游离体,正常标本来自6例意外截肢或身亡的新鲜膝关节软骨.方法:分离、体外培养两组关节软骨细胞.两组细胞分别给予100,500mg,L不同剂量的透明质酸钠干预及阴性对照(未加干预),培养6d.主要观察指标:通过反转录一聚合酶链反应观察透明质酸对大骨节病和正常人软骨细胞Ⅱ型胶原和聚集蛋白聚糖mRNA表达的影响.结果:大骨节病组软骨细胞Col Ⅱ和aggrecan mRNA表达量均较正常对照降低(P＜0.05),采用透明质酸钠补充治疗后,透明质酸钠100mg/L和500mg/L对大骨节病和正常人软骨细胞Col Ⅱ和aggrecan mRNA的表达均有促进作用,其中透明质酸钠500mg/L的促进作用更显著些.结论:补充透明质酸钠可以促进大骨节病软骨细胞Col Ⅱ和aggrecan的合成,其中,透明质酸钠500mg/L对大骨节病软骨细胞合成代谢的促进作用较透明质酸钠100mg/L明显.
배경:투명질산관절강주사치료골관절염이경성위일충상규방법,단대대골절병적치료환정재추엄,시가투명질산후대대골절병환자연골세포대사적영향,목전상위공백.목적:과제창신성설계관찰투명질산대체외배양적대골절병연골세포분비Ⅱ형효원화취집단백취당적영향,이기능지도림상치료.설계、시간급지점:대비관찰,실험우2006-09/2009-01재서안교통대학의학원생물의학연구실험중심화지방병실험실완성.재료:대골절관절병표본래원우6례대골절관절병환자실시슬관절수술적제적연골급유리체,정상표본래자6례의외절지혹신망적신선슬관절연골.방법:분리、체외배양량조관절연골세포.량조세포분별급여100,500mg,L불동제량적투명질산납간예급음성대조(미가간예),배양6d.주요관찰지표:통과반전록일취합매련반응관찰투명질산대대골절병화정상인연골세포Ⅱ형효원화취집단백취당mRNA표체적영향.결과:대골절병조연골세포Col Ⅱ화aggrecan mRNA표체량균교정상대조강저(P＜0.05),채용투명질산납보충치료후,투명질산납100mg/L화500mg/L대대골절병화정상인연골세포Col Ⅱ화aggrecan mRNA적표체균유촉진작용,기중투명질산납500mg/L적촉진작용경현저사.결론:보충투명질산납가이촉진대골절병연골세포Col Ⅱ화aggrecan적합성,기중,투명질산납500mg/L대대골절병연골세포합성대사적촉진작용교투명질산납100mg/L명현.
BACKGROUND:Intraarticular hyaluronic acid (HA)has been used as one common method in the treatment of osteoarthritis. But its application in treating Kashin-Beck disease (KBD) is generalizing. The effect of HA on chondrocyte metabolism in KBD patients remains unclear.OBJECTIVE:To investigate the effects of HA on the synthesis of collagen Ⅱ and aggrecan in KBD chondrocytes cultured in vitro to understand its clinical treatment for KBD.DESIGN,TIME AND SETTING:Comparative observation. The experiment was performed in the Laboratory Center of Biomedicine and Endemic Disease Laboratory of College of Medicine,Xi'an Jiaotong University from September 2006 to January 2009.MATERIALS:Samples of KBD were from cartilage and corpus liberum excised from 6 KBD patients undergoing knee joint operation,and normal samples of fresh knee cartilages were provided by 6 people undergoing amputation or death by accident METHODS:Chondrocytes of KBD and normal control were separated and cultured in vitro. Varying dosages of HA (100,500 gm/L) were administered to normal and KBD chondrocytes cultured in vitro for 6 days. Negative control was not treated with HA.MAIN OUTCOME MEASURES:Effects of HA on collagen Ⅱ and aggrecan mRNA expression in KBD patients and normal people by RT-PCR.RESULTS:Compared with normal control group,the mRNA level of collagen Ⅱ and aggrecan were lower in KBD (P＜0.05).After administration of HA (100,500 mg/L) the mRNA level of collagen Ⅱ and aggrecan were significantly increased,and the effects of 500 mg/L was more significant.CONCLUSION:HA can increase the collagen Ⅱ and aggrecan mRNA level of KBD chondrocytes cultured in vitro. In particular,500 mg/L HA significantly promoted chondrocyte metabolism compared with 100 mg/L HA.