CAJ | 학술논문

试验成功地培养了猪颗粒细胞,并采用激光共聚焦显微技术研究细胞有丝分裂过程中Ran的定位变化.结果表明,猪间期颗粒细胞中,Ran主要分布于细胞核内,核仁内没有Ran分布,细胞质中Ran浓度极低.核膜破裂,细胞进入有丝分裂,Ran分布到整个细胞,但在染色体周围浓集;在有丝分裂中期,浓集区形态和纺锤体的形态一致;在有丝分裂后期和末期,Ran的浓集区随纺锤体的拉长而分布于被分开的两组染色体之间;有丝分裂结束后,随着细胞核的形成,Ran在染色体周围浓集,核膜形成后又浓集于细胞核内,在整个有丝分裂中,Ran在细胞分裂区的细胞膜下未见特殊分布.这些分布与Ran在细胞周期中的作用是一致的.
시험성공지배양료저과립세포,병채용격광공취초현미기술연구세포유사분렬과정중Ran적정위변화.결과표명,저간기과립세포중,Ran주요분포우세포핵내,핵인내몰유Ran분포,세포질중Ran농도겁저.핵막파렬,세포진입유사분렬,Ran분포도정개세포,단재염색체주위농집;재유사분렬중기,농집구형태화방추체적형태일치;재유사분렬후기화말기,Ran적농집구수방추체적랍장이분포우피분개적량조염색체지간;유사분렬결속후,수착세포핵적형성,Ran재염색체주위농집,핵막형성후우농집우세포핵내,재정개유사분렬중,Ran재세포분렬구적세포막하미견특수분포.저사분포여Ran재세포주기중적작용시일치적.
Pig granular cells were successfully cultured and the localization and possible roles of Ran were investigated during the cell mitotic division by using the confocal laser scanning microscopy. The results showed that Ran was localized mainly in the nucleus, except the nucleolus, in the intermediate stage pig granular cell, whose density was very low in the cytoplasm. After the nuclear membrane broke down in mitochysis, Ran distributed throughout the cell, but it concentrated to the chromosome in mitosis metaphase, the concentrated area was uniformity to the spindle; in mitosis anaphase and telephase, the concentrated area localized between the separated chromosomes, but lower in other places. After mitosis, Ran coecentrated around the chromosome, and migrated into the forming nucleus. There is no special distribution for Ran under the membrane of the cell fission. These distributions were consistent with the roles of Ran in the cell cycle.