CAJ | 학술논문

以高羊茅(Festuca arundinacea Schrebb)品系宾哥(Bingo)为材料,建立了农杆菌介导的基因转化方法.高羊茅种子在9mg L-1 2,4-D作用下,获得胚性愈伤组织.愈伤组织经农杆菌侵染,在30和50 mg L-1的潮霉素浓度梯度下筛选,得抗性愈伤组织.组织化学染色法证实,uidA基因在抗性愈伤组织中表达;抗性愈伤组织在分化培养基中分化得到转基因植株.PCR、Southern杂交法证实外源基因已导入到高羊茅植株中.图5参37.
이고양모(Festuca arundinacea Schrebb)품계빈가(Bingo)위재료,건립료농간균개도적기인전화방법.고양모충자재9mg L-1 2,4-D작용하,획득배성유상조직.유상조직경농간균침염,재30화50 mg L-1적조매소농도제도하사선,득항성유상조직.조직화학염색법증실,uidA기인재항성유상조직중표체;항성유상조직재분화배양기중분화득도전기인식주.PCR、Southern잡교법증실외원기인이도입도고양모식주중.도5삼37.
Tall fescue (Festuca arundinacea Schreb.) is a cool-season turfgrass used on fairways in golf courses. The object of this study was to develop a more efficient, reliable, and repeatable approach in transforming the grass using Agrobacterium (EHA105), where β-glucuronidase gene (uidA) was used as a reporter and hygromycin phosphotransferase gene (hyg) as a selectable marker. An effective expression of transgene was observed in transforming 2-month-old calli derived from mature seeds (cv. Bingo) cultured on MS medium supused to obtain resistant calli. Transgenic plants have been produced from many independent transformed calli. The presence of functional β-glucuronidase gene (uidA) was detected in hygromycin-resistant calli. Transgenic plantswere regenerated and PCR and Southern blot confirmed transgene integration in the tall fescue genome.