CAJ | 학술논문

目的探讨应用临床型1.5T磁共振活体示踪磁标记猪骨髓间充质干细胞(MSCs)自体移植肝脏的可行性.方法获取、分离、培养、扩增猪骨髓MSCs.应用菲立磁标记细胞,普鲁士蓝染色鉴定,建立急性肝损伤模型,分为标记细胞组(n=6)和未标记细胞组(n=4)经门静脉行肝内移植,分别于移植前,移植后6 h、3 d、7 d、14 d行磁共振FFE(T_2WI)序列成像,14 d后行组织切片普鲁士蓝染色.结果普鲁士蓝染色表明MSCs的标记率达100%,磁标记MSCs肝内移植后行磁共振T_2WI序列呈明显低信号改变,并持续至细胞移植后14 d,组织切片普鲁士蓝染色显示14 d后仍有磁标记细胞存在于肝实质及肝窦中.结论利用菲立磁可以在体外成功标记猪骨髓间充质干细胞,磁共振成像可以对经门静脉移植到肝内的磁标记MSCs进行活体示踪.
목적 탐토응용림상형1.5T자공진활체시종자표기저골수간충질간세포(MSCs)자체이식간장적가행성.방법 획취、분리、배양、확증저골수MSCs.응용비립자표기세포,보로사람염색감정,건립급성간손상모형,분위표기세포조(n=6)화미표기세포조(n=4)경문정맥행간내이식,분별우이식전,이식후6 h、3 d、7 d、14 d행자공진FFE(T_2WI)서렬성상,14 d후행조직절편보로사람염색.결과 보로사람염색표명MSCs적표기솔체100%,자표기MSCs간내이식후행자공진T_2WI서렬정명현저신호개변,병지속지세포이식후14 d,조직절편보로사람염색현시14 d후잉유자표기세포존재우간실질급간두중.결론 이용비립자가이재체외성공표기저골수간충질간세포,자공진성상가이대경문정맥이식도간내적자표기MSCs진행활체시종.
Objective To evaluate in vivo tracking of swine mesenchymal stem cells (MSCs) la-beled with super paramagnetic iron oxide (SPIO) in intraportal transplantation by a clinical 1.5T MR.Methods MSCs were isolated from swine and cultured as well as expanded, which were then incuba-ted with SPIO (Feridex I. V.). Prussian blue staining was performed for showing intracelluar irons.To establish a swine model of acute liver necrosis, 0.5 g/kg of D-galactosamine was administrated to 10 pigs. MSCs(labeled cells in six, unlabeled cells in four)were injected into liver via portal veins. MR imaging was performed with a clinical 1.5T MR immediately before and at 6 h, 3 d, 7 d, 14 d after transplantation, respectively. Results Prussian blue staining of SPIO labeled MSCs could be effec-tively labeled and the labeling efficiency was almost 100%. Signal intensity loss in liver by SPIO labe-ling on FFE sequence persisted until 14 days after transplantation. Histological analysis by Prussian blue staining showed homing of labeled MSCs in liver after 14 days, primarily distributing in hepatic sinusoids and liver parenchyma. Conclusion MSCs can be labeled with SPIO in vitro successfully.MRI can monitor magnetically labeled MSCs transplanted into liver.