CAJ | 학술논문

目的了解谷胱甘肽转移酶M1(GSTM1)和T1(GSTT1)基因多态性在中国人群及吉林省结核涂阳人群中的分布.方法采用系统综述方法,以"GSTM1/GSTT1+多态性"为关键词搜索国内发表于2009年1月以前、研究类型为横断面研究或队列研究基线的文献,经综合分析获得GSTM1、GSTT1基因多态性分布信息.以吉林省14个县(区)2007年11月至2008年5月间的全部结核涂阳病例(共1120名)为研究对象,采用多重PCR法检测GSTM1、GSTT1基因型.结果系统综述得到中国人群GSTM1、GSTT1基因纯合缺失型和GSTM1-GSTT1联合缺失基因型频率分别为54.2%、46.8%和26.2%,其中以汉族为主的人群分别为53.4%、44.9%和25.5%;本研究中吉林省结核涂阳人群相应频率分别为57.2%、20.4%和13.7%,GSTM1、GSTT1基因型及组合基因型分布的性别、年龄差异无统计学意义(P>0.05).与系统综述结果相比,本研究人群GSTM1纯合缺失基因型频率偏高(P=0.016),GSTT1纯合缺失基因型和GSTM1-GSTT1联合缺失基因型频率明显偏低(P值均<0.001).结论 GSTM1、GSTT1基因多态性分布存在种族差异;本研究人群结果与系统综述结果的统计学差异可能是由于前者样本量较大、既往研究对象多为南方人群所致.
목적 료해곡광감태전이매M1(GSTM1)화T1(GSTT1)기인다태성재중국인군급길림성결핵도양인군중적분포.방법 채용계통종술방법,이"GSTM1/GSTT1+다태성"위관건사수색국내발표우2009년1월이전、연구류형위횡단면연구혹대렬연구기선적문헌,경종합분석획득GSTM1、GSTT1기인다태성분포신식.이길림성14개현(구)2007년11월지2008년5월간적전부결핵도양병례(공1120명)위연구대상,채용다중PCR법검측GSTM1、GSTT1기인형.결과 계통종술득도중국인군GSTM1、GSTT1기인순합결실형화GSTM1-GSTT1연합결실기인형빈솔분별위54.2%、46.8%화26.2%,기중이한족위주적인군분별위53.4%、44.9%화25.5%;본연구중길림성결핵도양인군상응빈솔분별위57.2%、20.4%화13.7%,GSTM1、GSTT1기인형급조합기인형분포적성별、년령차이무통계학의의(P>0.05).여계통종술결과상비,본연구인군GSTM1순합결실기인형빈솔편고(P=0.016),GSTT1순합결실기인형화GSTM1-GSTT1연합결실기인형빈솔명현편저(P치균<0.001).결론 GSTM1、GSTT1기인다태성분포존재충족차이;본연구인군결과여계통종술결과적통계학차이가능시유우전자양본량교대、기왕연구대상다위남방인군소치.
Objective To investigate the distribution of ghitathione-S-transferase M1 (GSTM1) and T1 (GSTT1) genes polymorphisms in Chinese population and smear-positive pulmonary tuberculosis cases of Jilin province. Methods Articles about GSTM1 and GSTT1 genes polymorphisms published before 2009 in China were searched. The study population was obtained from fourteen counties (or districts) of Jilin province, which included all cases from November, 2007 to May, 2008, totally 1120. The genotypes of GSTM1 and GSTT1 were detected by multiplex PCR technique. Results The frequencies of GSTM1 and GSTT1 'null' genotypes and combination M1-T1 'null' genotype acquired from systematic review were 54.2%, 46.8% and 26.2%, respectively, in Chinese Hans they were 53.4%, 44.9% and 25.5%, and in our research they are 57.2%, 20.4% and 13.7%, respectively. No significant differences between the frequencies of males and females as well as among that of different age groups were observed(P>0.05). The frequency of GSTM1 'null' genotype in our research is slightly higher than that in systematic review (P=0.016) , and the frequencies of GSTT1 'null' genotype and combination M1-T1 'null' genotype and are significantly lower than those in systematic review (both P<0.001). Conclusion The frequencies of GSTM1 and GSTTI 'null' genotypes were different among ethnics. The statistical difference between systematic review and our research may due to our large sample size and mostly Soutbern people in previous studies.