中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
11期
1231-1234
,共4页
叶景荣%卢红艳%白立石%辛若雷%曾毅
葉景榮%盧紅豔%白立石%辛若雷%曾毅
협경영%로홍염%백립석%신약뢰%증의
人类免疫缺陷病毒%亚型%巢式聚合酶链式反应
人類免疫缺陷病毒%亞型%巢式聚閤酶鏈式反應
인류면역결함병독%아형%소식취합매련식반응
Human immunodeficiency virus%Subtype%Nested-PCR
目的 分析北京地区HIV-1 CRF01 AE株在的基因特征.方法 采集北京地区2006-2008年新确证HIV感染者的抗凝全血标本,分离血浆和提取病毒RNA,用反转录/巢式聚合酶链式反应扩增病毒gag基因,对获得的105条CRF01_AE株序列进行系统进化分析,并通过计算组间距离和Entropy核苷酸多态性差异方法分析病毒株的遗传特征.结果 在CRF01 AE株序列系统进化树中存在4个主要的亚簇,Homo-Max亚簇包含67例样本,同参考株CM240组间基因 离散率为5.4%,组内离散率为2.8%;Hetero亚簇包含6例样本,组间基因离散率为4.8%,组内离散率为4.2%.Mix亚簇包含8例样本,组间基因离散率为4.2%,组内离散率为5.2%.Homo-Min亚簇包含18例样本,组间基因离散率为4.4%,组内离散率为2.8%.Homo-Max、Hetero和Homo-Min亚簇无其他国家的序列,Mix亚簇与越南和泰国CRF01 AE株关系密切;核苷酸多态性分析结果显示,以Mix亚簇为参照,Homo-Max、Homo-Min和Hetero亚簇分别有37、29和11个位点的核苷酸组成,差异有统计学意义.结论 北京地区流行的CRF01_AE病毒株中首次观察到4个独立的亚簇;Homo-Max亚簇株为最主要的CRF01_AE株;Mix亚簇株与来自于泰国和越南的CRF01 AE株有较高同源性.
目的 分析北京地區HIV-1 CRF01 AE株在的基因特徵.方法 採集北京地區2006-2008年新確證HIV感染者的抗凝全血標本,分離血漿和提取病毒RNA,用反轉錄/巢式聚閤酶鏈式反應擴增病毒gag基因,對穫得的105條CRF01_AE株序列進行繫統進化分析,併通過計算組間距離和Entropy覈苷痠多態性差異方法分析病毒株的遺傳特徵.結果 在CRF01 AE株序列繫統進化樹中存在4箇主要的亞簇,Homo-Max亞簇包含67例樣本,同參攷株CM240組間基因 離散率為5.4%,組內離散率為2.8%;Hetero亞簇包含6例樣本,組間基因離散率為4.8%,組內離散率為4.2%.Mix亞簇包含8例樣本,組間基因離散率為4.2%,組內離散率為5.2%.Homo-Min亞簇包含18例樣本,組間基因離散率為4.4%,組內離散率為2.8%.Homo-Max、Hetero和Homo-Min亞簇無其他國傢的序列,Mix亞簇與越南和泰國CRF01 AE株關繫密切;覈苷痠多態性分析結果顯示,以Mix亞簇為參照,Homo-Max、Homo-Min和Hetero亞簇分彆有37、29和11箇位點的覈苷痠組成,差異有統計學意義.結論 北京地區流行的CRF01_AE病毒株中首次觀察到4箇獨立的亞簇;Homo-Max亞簇株為最主要的CRF01_AE株;Mix亞簇株與來自于泰國和越南的CRF01 AE株有較高同源性.
목적 분석북경지구HIV-1 CRF01 AE주재적기인특정.방법 채집북경지구2006-2008년신학증HIV감염자적항응전혈표본,분리혈장화제취병독RNA,용반전록/소식취합매련식반응확증병독gag기인,대획득적105조CRF01_AE주서렬진행계통진화분석,병통과계산조간거리화Entropy핵감산다태성차이방법분석병독주적유전특정.결과 재CRF01 AE주서렬계통진화수중존재4개주요적아족,Homo-Max아족포함67례양본,동삼고주CM240조간기인 리산솔위5.4%,조내리산솔위2.8%;Hetero아족포함6례양본,조간기인리산솔위4.8%,조내리산솔위4.2%.Mix아족포함8례양본,조간기인리산솔위4.2%,조내리산솔위5.2%.Homo-Min아족포함18례양본,조간기인리산솔위4.4%,조내리산솔위2.8%.Homo-Max、Hetero화Homo-Min아족무기타국가적서렬,Mix아족여월남화태국CRF01 AE주관계밀절;핵감산다태성분석결과현시,이Mix아족위삼조,Homo-Max、Homo-Min화Hetero아족분별유37、29화11개위점적핵감산조성,차이유통계학의의.결론 북경지구류행적CRF01_AE병독주중수차관찰도4개독립적아족;Homo-Max아족주위최주요적CRF01_AE주;Mix아족주여래자우태국화월남적CRF01 AE주유교고동원성.
Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in Beijing. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals being reported during 2006 to 2008 in Beijing. Gag gene fragments were amplified from RNA template which were extracted from plasma by RT and nested PCR methods. 105 CRF01_AE sequences were analyzed by phylogcnetic methods and characterized through calculating the genetic distance and Entropy analysis. Results There were four main sub-clusters in the phylogenetic tree.We named them as sub-clusters Homo-Max ( 67 sequences ), Hetero( 6 sequences), Mix (8 sequences)and Homo-Min ( 18 sequences)respectively, based on the mode of transmission. It was found that no international reference strain was closely related to the sub-cluster Homo-Max, Hetero or Homo-Min,including 91 samples. The strains in sub-cluster Mix consisting 8 cases that were closely related to the strains identified in Thailand and Vietnam. Genetic distance analysis on gag genes showed that the diversity of sub-clusters Homo-Max and Homo-Min was obviously less than that of the sub-cluster Hetero or Mix. When compared with sub-cluster Mix, there were 37,29 and 11 significantly different nucleotides polymorphism compositions sites in sub-clusers Homo-Max Homo-Min and Hetero.Conclusion This was the first report describing that four main epidemic sub-clusters were existed in CRF01_AE strains prevailing in Beijing. The virus with sub-cluster Homo-Max was the dominant strain in this region with shorter period of circulation and higher proportion seen in the HIV-infected persons. The virus in sub-cluster Mix was highly homologic with the CRF01_AE strains from Thailand and Vietnam.