CAJ | 학술논문

目的观察高糖(培养)对离体雪旺细胞活性和增殖能力的影响,探讨周围神经再生时糖尿病性损伤的可能机制.方法采用brockes改良法从新生Wistar乳鼠坐骨神经组织中分离纯化雪旺细胞.实验分为三组:采用正糖(含5 mmol/L葡萄糖)为对照组.高糖(含30 mmol/L葡萄糖)为高糖组,高糖高渗(含30 mmol/L葡萄糖+100 mmol NaCI)为高渗组;每组6对.采用XTT法测定雪旺细胞活性,3H-TdR掺人法检测雪旺细胞的增殖能力.结果高糖组、高渗组同对照组比较,细胞活力明显减少.差异有统计学意义(P＜0.05);高糖组与对照组比较,细胞增殖能力受到抑制,两组差异有统计学意义(P＜0.01);高渗组与高糖组比较,细胞增殖能力明显受到抑制,差异有明显统计学意义(P＜0.01).表明高糖对离体雪旺细胞活性和增殖能力均具有显著抑制作用,但较高渗同时存在时为轻.结论周围神经再生时糖尿病性损伤可能与高糖状态影响雪旺细胞的活力和增殖能力有关.
목적 관찰고당(배양)대리체설왕세포활성화증식능력적영향,탐토주위신경재생시당뇨병성손상적가능궤제.방법 채용brockes개량법종신생Wistar유서좌골신경조직중분리순화설왕세포.실험분위삼조:채용정당(함5 mmol/L포도당)위대조조.고당(함30 mmol/L포도당)위고당조,고당고삼(함30 mmol/L포도당+100 mmol NaCI)위고삼조;매조6대.채용XTT법측정설왕세포활성,3H-TdR참인법검측설왕세포적증식능력.결과 고당조、고삼조동대조조비교,세포활력명현감소.차이유통계학의의(P＜0.05);고당조여대조조비교,세포증식능력수도억제,량조차이유통계학의의(P＜0.01);고삼조여고당조비교,세포증식능력명현수도억제,차이유명현통계학의의(P＜0.01).표명고당대리체설왕세포활성화증식능력균구유현저억제작용,단교고삼동시존재시위경.결론 주위신경재생시당뇨병성손상가능여고당상태영향설왕세포적활력화증식능력유관.
Objective To examine the effect of culture with high glucose on activity and proliferation of Schwann cells and to investigate the possible mechanism of diabetic lesion in peripheral nerve regeneration. Methods Schwann cells were isolated from sciatic nerves of newborn Wistar suckling mrs and purified using modified Brockes method. The cells were then cultured under various conditions according to group assignment: control group (normo-glucose, 5 mmol/L glucose), hyper-glucose group (30 mmol/L glucose), and hyperosmotic group(30 mmol/L glucose + 100 mmol NaCI). XTT methed and 3H-TdR incorporative method were adopted to measure the activity and proliferation ability of Schwann cells. Results As compared with the control group, the cellular activity in the hyper-glucese and hyper-osmotic group was markedly attenuated (P＜ O.05). Proliferation ability of the Schwann cells in the hyper- glucose group was markedly inhibited (P ＜ 0.01) as compare to those in the control group. Proliferation ability of the Schwann ceils in the hyper-osmotic group was even lower than that of the hyper-glucose group, indicating that high glucose had a marked inhibitory effect on the activity and proliferation ability of Schwann cells. The presence of hyperosmotic state further attenuated cell activity and proliferation. Conclusion Diabetic lesion in peripheral nerve regeneration is likely related to the inhibitory effect of high glucose on the activity and proliferation ability of Schwann cells.