CAJ | 학술논문

从水稻(Oryza sativa L.)、烟草(Nicotiana tabacum L.)和黑松(Pinus thunbergiiParl.)等植物的22对叶绿体SSR引物中筛选出 5对能用于柑橘叶绿体SSR分析的引物,应用这5对引物对9个组合的柑橘体细胞杂种的叶绿体遗传进行了分析.结果表明:这些组合再生的杂种中叶绿体都呈现随机分离,该现象与以前报道的RFLP分析结果一致,而且其可靠性已被CAPS分析所证实.表明柑橘叶绿体SSR同RFLP及CAPS一样可靠,并且更简单高效、易于操作,特别适合对柑橘等植物体细胞杂种进行早期胞质遗传组成分析.
종수도(Oryza sativa L.)、연초(Nicotiana tabacum L.)화흑송(Pinus thunbergiiParl.)등식물적22대협록체SSR인물중사선출 5대능용우감귤협록체SSR분석적인물,응용저5대인물대9개조합적감귤체세포잡충적협록체유전진행료분석.결과표명:저사조합재생적잡충중협록체도정현수궤분리,해현상여이전보도적RFLP분석결과일치,이차기가고성이피CAPS분석소증실.표명감귤협록체SSR동RFLP급CAPS일양가고,병차경간단고효、역우조작,특별괄합대감귤등식물체세포잡충진행조기포질유전조성분석.
Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and success-fully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previouslyreported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Oryza sativa L.) and tobacco (Nicotianatabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gelelectrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was thenanalyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gelelectrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzedCitrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. Itwas also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids inhigher plants, compared with the conventional RFLP or CAPS analyses.