CAJ | 학술논문

利用间接免疫萎光标记技术研究Ser10磷酸化的组蛋白H3和微管蛋白在小麦根尖细胞中有丝分裂过程中的动态分布情况.结果显示在小麦根尖细胞有丝分裂过程中Ser10磷酸化的组蛋白H3的出现和消失与染色体的凝集和解凝集的过程存在时空上的相关性,在有丝分裂的过程中这种蛋白在着经线粒上的定位有有助于染色体向两极移动.研究结果还表明,在有丝分裂过程中,微管蛋白发生了重组,成束的垂直排列在赤道板的两侧,协助细胞有丝分裂过程的顺利完成.
이용간접면역위광표기기술연구Ser10린산화적조단백H3화미관단백재소맥근첨세포중유사분렬과정중적동태분포정황.결과현시재소맥근첨세포유사분렬과정중Ser10린산화적조단백H3적출현화소실여염색체적응집화해응집적과정존재시공상적상관성,재유사분렬적과정중저충단백재착경선립상적정위유유조우염색체향량겁이동.연구결과환표명,재유사분렬과정중,미관단백발생료중조,성속적수직배렬재적도판적량측,협조세포유사분렬과정적순리완성.
The indirect immunofluorescence labeling tehnology was used to study the dynamic distribution of Ser-10 phosphorylated histone H3 and microtubules in wheat root cells during the mitosis.The result showed that during mitotic division,the Ser-10 phosphorylated histone H3 start from the prophase and vanished at telophase,remaining mainly in the pericentromeric regions at metaphase.During the mitosis the microtubules take on regulated change pattern.May be during the mitosis the Ser-10 phosphorylated histone H3 is the centromere/kinetochore and responsible for interact with microtubules and assist the chromosome movement.