中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2008年
40期
7968-7970
,共3页
闫朝岐%邹小明%李刚%宋茂力%李晓林%李云龙%杨春发%李莹杰%王怀泉%周亚滨
閆朝岐%鄒小明%李剛%宋茂力%李曉林%李雲龍%楊春髮%李瑩傑%王懷泉%週亞濱
염조기%추소명%리강%송무력%리효림%리운룡%양춘발%리형걸%왕부천%주아빈
猪%小肠移植%围手术期
豬%小腸移植%圍手術期
저%소장이식%위수술기
实验于2004 02/2006-05在哈尔滨医科大学附属第二医院完成.杂种小猪40只,供体20只,受体20只.[1]供体手术:原位腹主动脉4℃肝素盐水500 mL灌洗,灌洗压力为7.8～9.8 kPa,快速切除供体肠管后置于4℃生理盐水中保存.[2]受体手术:根据探查情况,分别行原位和异位小肠移植.40～45℃生理盐水冲洗腹腔,直至吸引出的冲洗液变温暖为止.[3]围手术期管理:移植前、移植后讨论,注意体温的监测和控制,尝试早期进食,其中4只移植后自由进水,第2天给予正常饮食,其余移植后2 d进水.[4]移植后评估:实验动物进食后无吻合口漏发生.16只存活超过7 d,最长存活超过14 d.平均存活9 d,小肠移植手术成功率为80%(16/20).失败原因:静脉血栓1只,失血1只,其他原因2只.结果提示开展的围手术期管理经验经过大量的小肠移植实践取得了较为满意的效果.
實驗于2004 02/2006-05在哈爾濱醫科大學附屬第二醫院完成.雜種小豬40隻,供體20隻,受體20隻.[1]供體手術:原位腹主動脈4℃肝素鹽水500 mL灌洗,灌洗壓力為7.8～9.8 kPa,快速切除供體腸管後置于4℃生理鹽水中保存.[2]受體手術:根據探查情況,分彆行原位和異位小腸移植.40～45℃生理鹽水遲洗腹腔,直至吸引齣的遲洗液變溫暖為止.[3]圍手術期管理:移植前、移植後討論,註意體溫的鑑測和控製,嘗試早期進食,其中4隻移植後自由進水,第2天給予正常飲食,其餘移植後2 d進水.[4]移植後評估:實驗動物進食後無吻閤口漏髮生.16隻存活超過7 d,最長存活超過14 d.平均存活9 d,小腸移植手術成功率為80%(16/20).失敗原因:靜脈血栓1隻,失血1隻,其他原因2隻.結果提示開展的圍手術期管理經驗經過大量的小腸移植實踐取得瞭較為滿意的效果.
실험우2004 02/2006-05재합이빈의과대학부속제이의원완성.잡충소저40지,공체20지,수체20지.[1]공체수술:원위복주동맥4℃간소염수500 mL관세,관세압력위7.8～9.8 kPa,쾌속절제공체장관후치우4℃생리염수중보존.[2]수체수술:근거탐사정황,분별행원위화이위소장이식.40～45℃생리염수충세복강,직지흡인출적충세액변온난위지.[3]위수술기관리:이식전、이식후토론,주의체온적감측화공제,상시조기진식,기중4지이식후자유진수,제2천급여정상음식,기여이식후2 d진수.[4]이식후평고:실험동물진식후무문합구루발생.16지존활초과7 d,최장존활초과14 d.평균존활9 d,소장이식수술성공솔위80%(16/20).실패원인:정맥혈전1지,실혈1지,기타원인2지.결과제시개전적위수술기관리경험경과대량적소장이식실천취득료교위만의적효과.
The experiment was conducted between February 2004 and May 2006 at The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang province, China. Forty hybrid pigs were used in the experiment, 20 were used as donors and the other 20 were used as recipients. Donor operation: Abdominal aorta was lavaged in situ with 500 mL 4℃ heparinized saline (lavage pressure 7.8-9.8 kPa), intestinal canal cut from donor was conserved in 4℃ physiological saline.Recipient operation: Orthotopic small intestine transplantation and heterotopic small intestine transplantation were conducted according to exploring results, and abdominal cavity was washed with 40-45℃ physiological saline until the flush out liquid became warm.Periprocedural management: Preoperative discussion and postoperative discussion were done, and body temperature of pigs was monitoring and controlled. Early-stage feeding was used, 4 pigs could drink water freely after transplantation and took food normally at the second day, while other 16 pigs began to drink water 2 days after transplantation. Evaluation after the operation: No experimental animal had anastomotic leakage after taking food. Sixteen pigs survived over 7 days, average 9 days, the longest over 14 days. The success rate of the small intestine transplantation is 80% (16/20). Failure reasons: One with phlebothrombesis, one with blood loss and two with unknown reasons. Results showed that experience of periprocedural management had got relatively satisfactory effects through a great quantity of small intestine transplantation practices.
