CAJ | 학술논문

背景:葛根异黄酮具有类雌二醇结构特征,对去卵巢大鼠的骨质丢失和骨量的减少有一定的抑制作用.但也有报道其对去卵巢大鼠骨质疏松的防治效果较弱且单独使用效果不明显.目的:观察葛根异黄酮与维生素D联合对去卵巢大鼠骨质疏松的作用.方法:将81只3月龄雌性SD大鼠随机分为9组:除假手术组外其他各组均行双侧卵巢摘除造模.低、中、高剂量TIP组,VitD组,低、中、高剂量联合组在去卵巢基础上分别灌胃给予相应剂量的葛根异黄酮和(或)维生素D;模型组和假手术组不给予药物治疗.给药3个月,计算大鼠子宫系数;测定大鼠血清碱性磷酸酶、血钙、血磷、骨钙素及血清雌二醇水平;检测大鼠股骨骨密度.结果与结论:与模型组比较,中、高剂量TIP组,低、中、高剂量联合组大鼠子宫系数、雌二醇水平明显增高(P < 0.05),血清碱性磷酸酶、骨钙素水平明显降低(P < 0.05),股骨中心及远心骨密度均明显增加(P < 0.05),均以高剂量联合组作用效果最明显.且在子宫系数,雌二醇、血清碱性磷酸酶、骨钙素水平及股骨远心端骨密度方面,葛根异黄酮和维生素D均表现为协同作用.在血钙、血磷方面,葛根异黄酮和(或)维生素D的作用不明显.说明葛根异黄酮和维生素D联合使用对去卵巢大鼠骨质疏松的防治有协同效应.
배경:갈근이황동구유류자이순결구특정,대거란소대서적골질주실화골량적감소유일정적억제작용.단야유보도기대거란소대서골질소송적방치효과교약차단독사용효과불명현.목적:관찰갈근이황동여유생소D연합대거란소대서골질소송적작용.방법:장81지3월령자성SD대서수궤분위9조:제가수술조외기타각조균행쌍측란소적제조모.저、중、고제량TIP조,VitD조,저、중、고제량연합조재거란소기출상분별관위급여상응제량적갈근이황동화(혹)유생소D;모형조화가수술조불급여약물치료.급약3개월,계산대서자궁계수;측정대서혈청감성린산매、혈개、혈린、골개소급혈청자이순수평;검측대서고골골밀도.결과여결론:여모형조비교,중、고제량TIP조,저、중、고제량연합조대서자궁계수、자이순수평명현증고(P < 0.05),혈청감성린산매、골개소수평명현강저(P < 0.05),고골중심급원심골밀도균명현증가(P < 0.05),균이고제량연합조작용효과최명현.차재자궁계수,자이순、혈청감성린산매、골개소수평급고골원심단골밀도방면,갈근이황동화유생소D균표현위협동작용.재혈개、혈린방면,갈근이황동화(혹)유생소D적작용불명현.설명갈근이황동화유생소D연합사용대거란소대서골질소송적방치유협동효응.
BACKGROUND: Total isoflavones of pueraria (TIP) possesses estradiol-like structures and has inhibition effect on bone loss or osteopenia in ovariectomized rats. However, studies have demonstrated that the prevention and treatment effect of TIP on osteoporosis in ovariectomized rats is poor, especially in single utilization. OBJECTIVE: To investigate the combination effect of TIP and vitamin D on the treatment of osteoporosis in ovariectomized rats. METHODS: Totally 81 female sprague-drawly (SD) rats of 3-month-old were randomly assigned into 9 groups. Exception those in the sham-surgery group, all rats were prepared for ovariectomized models. Vitamin D or low-, middle- and high-dose TIP or low-, middle- and high-dose TIP combined with vitamin D were intragastric administrated in the vitamin D, TIP or combination groups, respectively. There was no drug medication in the model and sham-surgery groups. The uterus coefficient was calculated at 3 months after medication. The serum alkaline phosphatase (ALP) activity, calcium, phosphonium, bone gla protein, estradiol levels, as well as bone mineral density of femur was determined. RESULTS AND CONCLUSION: Compared with the model group, the uterus coefficient and estradiol level was obviously increased in the middle- and high-dose TIP groups and all combination groups (P < 0.05); the ALP and bone gla protein levels significant decreased (P < 0.05); and the bone mineral density of central and distal femur were notably increased (P < 0.05); in particular, the result was more manifest in the high-dose combination group. TIP and vitamin D presented with synergism in uterus coefficient, estradiol, ALP, bone gla protein levels and bone mineral density of distal femur, but the effect was not significant in the calcium and phosphonium levels in the TIP and/or vitamin D groups. The findings demonstrated that Tip combined with vitamin D can induce synergism on prevention and cure of osteporosis in ovariectomized rat.