国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2010年
9期
588-591
,共4页
宫晓光%吕云福%岳劼%邱庆安
宮曉光%呂雲福%嶽劼%邱慶安
궁효광%려운복%악할%구경안
阻塞性黄疸%α-硫辛酸%能量代谢%丙二醛%过氧化物歧化酶
阻塞性黃疸%α-硫辛痠%能量代謝%丙二醛%過氧化物歧化酶
조새성황달%α-류신산%능량대사%병이철%과양화물기화매
Obstructive jaundice%α-lipoic acid%Energy metabolism%Malondialdehyde%Superoxidedismutase
目的 探讨α-硫辛酸对阻塞性黄疸大鼠肝细胞线粒体内能量代谢的保护作用及其机制. 方法 将72只SD雄性大鼠随机分为对照组(SO组),胆总管结扎+0.9%氯化钠溶液组(BDL+NS组),胆总管结扎α-硫辛酸组(BDL+LA组).分别于术后7 d、14 d和2l d检测肝细胞线粒体内MDA、SOD、ATP、ADP和AMP含量并计算总腺苷酸(TAN)和能荷(EC). 结果 BDL+NS组各时间点肝细胞线粒体内MDA、ADP、AMP含量明显升高,而SOD、ATP、EC含量却明显降低.胆总管结扎7 d、14 d时,肝细胞线粒体内MDA含量BDL+LA组比BDL+NS组低,差异有统计学意义(P＜0.01);2l d时,AMP、MDA含量BDL+NS组和BDL+LA中都更进一步升高,但两组比较差异无统计学意义(P＞0.05).胆总管结扎7 d、14 d时,肝细胞线粒体内SOD含量、ATP含量BDL+LA组比BDL+NS组下降程度有显著性差异(7 d,P＜0.01;14 d,P＜0.05);21 d时,两组肝细胞线粒体内SOD含量都进一步下降,但差异无统计学意义(p＞0.05). 结论 α-硫辛酸在阻塞性黄疸早、中期有保护线粒体能量代谢作用,减轻了阻塞性黄疸时肝的损伤.
目的 探討α-硫辛痠對阻塞性黃疸大鼠肝細胞線粒體內能量代謝的保護作用及其機製. 方法 將72隻SD雄性大鼠隨機分為對照組(SO組),膽總管結扎+0.9%氯化鈉溶液組(BDL+NS組),膽總管結扎α-硫辛痠組(BDL+LA組).分彆于術後7 d、14 d和2l d檢測肝細胞線粒體內MDA、SOD、ATP、ADP和AMP含量併計算總腺苷痠(TAN)和能荷(EC). 結果 BDL+NS組各時間點肝細胞線粒體內MDA、ADP、AMP含量明顯升高,而SOD、ATP、EC含量卻明顯降低.膽總管結扎7 d、14 d時,肝細胞線粒體內MDA含量BDL+LA組比BDL+NS組低,差異有統計學意義(P＜0.01);2l d時,AMP、MDA含量BDL+NS組和BDL+LA中都更進一步升高,但兩組比較差異無統計學意義(P＞0.05).膽總管結扎7 d、14 d時,肝細胞線粒體內SOD含量、ATP含量BDL+LA組比BDL+NS組下降程度有顯著性差異(7 d,P＜0.01;14 d,P＜0.05);21 d時,兩組肝細胞線粒體內SOD含量都進一步下降,但差異無統計學意義(p＞0.05). 結論 α-硫辛痠在阻塞性黃疸早、中期有保護線粒體能量代謝作用,減輕瞭阻塞性黃疸時肝的損傷.
목적 탐토α-류신산대조새성황달대서간세포선립체내능량대사적보호작용급기궤제. 방법 장72지SD웅성대서수궤분위대조조(SO조),담총관결찰+0.9%록화납용액조(BDL+NS조),담총관결찰α-류신산조(BDL+LA조).분별우술후7 d、14 d화2l d검측간세포선립체내MDA、SOD、ATP、ADP화AMP함량병계산총선감산(TAN)화능하(EC). 결과 BDL+NS조각시간점간세포선립체내MDA、ADP、AMP함량명현승고,이SOD、ATP、EC함량각명현강저.담총관결찰7 d、14 d시,간세포선립체내MDA함량BDL+LA조비BDL+NS조저,차이유통계학의의(P＜0.01);2l d시,AMP、MDA함량BDL+NS조화BDL+LA중도경진일보승고,단량조비교차이무통계학의의(P＞0.05).담총관결찰7 d、14 d시,간세포선립체내SOD함량、ATP함량BDL+LA조비BDL+NS조하강정도유현저성차이(7 d,P＜0.01;14 d,P＜0.05);21 d시,량조간세포선립체내SOD함량도진일보하강,단차이무통계학의의(p＞0.05). 결론 α-류신산재조새성황달조、중기유보호선립체능량대사작용,감경료조새성황달시간적손상.
Objective To investigate the effects of α-lipoic acid on mitochondrial energy metabolism of liver in the rat with obstructive jaundice. Methods Seventy-two male SD rats were randomly divided into groups of sham operation and bile duct-ligation + normal soldium( BDL + NS) and bileduct-ligation + α-lipoic acid( BDL + LA). Malondialdehyde(MDA) , superoxide dismutase(SOD) and ATP, ADP, AMP in rat liver's mitochondrion were examined on days 7,14,21 after operation. Results MDA contents, ADP contents and AMP contents of liver' s mitochondrion were significantly increased at each time point in BDL + NS-group,while SOD contents and ATP contents of liver' s mitochondrion decreased remarkably on the 7th, 14th days; MDA content of liver' s mitochondrion in BDL + LA group was more lower than that in BDL + NS group (P ＜ 0. 01 )on the 21st day; MDA content was further increased in BDL + NS group and in BDL + LA group but there was no difference in their comparisons. On the 7th, 14th days, SOD contents and ATP contents in BDL + LA group were significantly higher than that in BDL + NS group( 7th days,P ＜ 0. 01; 14th days,P ＜ 0.05 ), On the 21 st day SOD contents in BDL + NS group had no difference with that in BDL + LA group (P ＞ 0. 05). Conclusions LA has a protective effect on energy metabolism of liver' s mitochondrion in the initial ,metaphase stage of obstructive jaundice.
