CAJ | 학술논문

目的针对现行尿碘检测方法中测定误差因素多的特点,建立实验室内质量控制体系,确保检验数据的准确可靠.方法收集日常检测中空白管吸光度值及标准物质检测值,计算均值、标准偏差、控制限、辅助线,以它们为线绘制空白管吸光度值控制图及相对误差控制图;用不同碘含量(高、中、低浓度)的试样测定结果,计算各组平均值、相对减差值、加权值、临界限(Rc)值,列出临界限Rc值控制表.结果空白值吸光度控制限((x)±3sb)范围为1.183～1.553;精密度控制临界限Rc值在尿碘0-<150 μg/L时为0.0883,150～300 μg/L时为0.0572;相对误差控制限(E+3se)为9.3%.结论建立质量控制图可有效控制实验室内尿碘检测质量,保证尿碘测定结果的可靠性和真实性.
목적 침대현행뇨전검측방법중측정오차인소다적특점,건립실험실내질량공제체계,학보검험수거적준학가고.방법 수집일상검측중공백관흡광도치급표준물질검측치,계산균치、표준편차、공제한、보조선,이타문위선회제공백관흡광도치공제도급상대오차공제도;용불동전함량(고、중、저농도)적시양측정결과,계산각조평균치、상대감차치、가권치、림계한(Rc)치,렬출림계한Rc치공제표.결과 공백치흡광도공제한((x)±3sb)범위위1.183～1.553;정밀도공제림계한Rc치재뇨전0-<150 μg/L시위0.0883,150～300 μg/L시위0.0572;상대오차공제한(E+3se)위9.3%.결론 건립질량공제도가유효공제실험실내뇨전검측질량,보증뇨전측정결과적가고성화진실성.
Objective Establish a laboratory quality control system to ensure accurate and reliable test data and to contrapose the influence of error factors in current detection methods for urinary iodine measurement. Methods The results of reagent blank absorbance value and uric iodine standard materials were collected, then their relevant technical indexes such as mean, standard deviation, control limit, auxiliary line were worked out. Then the quality control chart of blank test and the relative error control chart were made base on these technical indexes. And different iodine concentrations (high, middle and low concentration) were tested and their mean,relative reduction difference value, weighted mean value and critical limit Rc value were calculated, and then critical limit Rc value precision control chart was made. Results The range of absorbance of blank control test was 1.183 to 1.553. And the limit of the accuracy control Rc value was 0.0883, 0.0572, respectively, when the concentrations of urinary iodine was 0～ < 150 μg/L and 150 ～ 300 μg/L. The control limit of the relative error was 9.3%. Conclusions The method of quality control chart could be satisfactorily applied to identify the quality of the analytical results of urine iodine, and ensure the results of the urine iodine reliable and authentic.