CAJ | 학술논문

目的观察Smad4条件基因敲除小鼠内耳前庭的组织学变化,探讨Smad4基因在内耳前庭发育中的作用.方法应用冰冻切片、免疫荧光化学、激光扫描共聚焦显微镜、扫描电镜、透射电镜等技术,观察软骨组织Smad4条件基因敲除小鼠内耳前庭组织形态的变化.结果 Smad4 -/-小鼠内耳软骨囊Smad4免疫反应阴性,Smad4+/-小鼠阳性,Smad4+/+小鼠强阳性,而在前庭感觉上皮——壶腹嵴和囊斑里Smad4免疫反应均呈阳性,三个基因型间没有差别；通过扫描电镜和透射电镜以及神经丝免疫荧光染色观察,三个基因型小鼠内耳前庭均未发现病变.结论 Smad4条件基因敲除小鼠的内耳软骨囊里Smad4基因被有效剔除,但其对内耳前庭的组织结构和形态影响有限.
목적 관찰Smad4조건기인고제소서내이전정적조직학변화,탐토Smad4기인재내이전정발육중적작용.방법 응용빙동절편、면역형광화학、격광소묘공취초현미경、소묘전경、투사전경등기술,관찰연골조직Smad4조건기인고제소서내이전정조직형태적변화.결과 Smad4 -/-소서내이연골낭Smad4면역반응음성,Smad4+/-소서양성,Smad4+/+소서강양성,이재전정감각상피——호복척화낭반리Smad4면역반응균정양성,삼개기인형간몰유차별；통과소묘전경화투사전경이급신경사면역형광염색관찰,삼개기인형소서내이전정균미발현병변.결론 Smad4조건기인고제소서적내이연골낭리Smad4기인피유효척제,단기대내이전정적조직결구화형태영향유한.
Objective To investigate the histological changes in the vestibular endorgans of Smad4 gene conditional knockout mice and to explore the influence of the Smad4 gene on vestibular development.Methods Histological changes of periphery vestibular organs in inner ear of Smad4 conditional knockout mice were investigated by frozen sections,immunofluorescence,confocal microscopy,scanning electron microscopy and transmission electron microscopy.Results There was no Smad4 expression in the inner ear cartilage capsule of Smad4 -/- mice.In Smad4 +/- mice,Smad4 expression in the same cartilage capsule was positive,and it was strong positive in Smad4 +/+ mice.Smad4 expression in vestibular sense epithelium,crista ampullaris and macula,was positive.And no difference was found among these three genotypes.Studying at scanning electron microscopy and transmission electron microscopy levels and antifilament immunofluorescence showed that no pathological changes were observed in all the three genotype mice.Conclusion Although the Smad4 gene was knockout effectively in the auricular cartilage capsule of Smad4 conditional knockout mice,the histological changes of Smad4 conditional knockout mice in vestibulum auris internae were slightly.