中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2008年
7期
434-436
,共3页
朱庆党%巢永烈%陈新民%HU Jun
硃慶黨%巢永烈%陳新民%HU Jun
주경당%소영렬%진신민%HU Jun
成纤维细胞%应力,物理%胶原Ⅰ型%纤连蛋白类
成纖維細胞%應力,物理%膠原Ⅰ型%纖連蛋白類
성섬유세포%응력,물리%효원Ⅰ형%섬련단백류
Fibroblasts%Stress,mechanical%Collagen type Ⅰ%Fibronectins
目的 探讨机械应力下人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLF)Ⅰ型胶原和纤连蛋白mRNA表达的变化,为阐明力信号的细胞内转导机制提供依据. 方法 对体外培养的hPDLF分别施加不同动态张、压应力(强度为1000、2000、4000 μstrain,加力时间为0、0.5、1、4、8、12 h),定量聚合酶链反应(PCR)检测Ⅰ型胶原和纤连蛋白mRNA表达. 结果 随时间延长1000 μstrain张应力和压应力均使Ⅰ型胶原和纤连蛋白mRNA表达增强,2000 μstrain和4000 μstrain张应力均使Ⅰ型胶原mRNA表达先增强1 h后减弱,而2000 μstrain和4000μstrain压应力均使Ⅰ型胶原mRNA表达持续减弱,2000 μstrain张应力和压应力均使纤连蛋白mRNA表达持续增强,而4000 μstrain张应力和压应力均使纤连蛋白mRNA表达先增强4 h后减弱. 结论 本项实验提供的应力范围内动态张应力和压应力刺激可改变hPDLF Ⅰ型胶原和纤连蛋白mRNA的表达.
目的 探討機械應力下人牙週膜成纖維細胞(human periodontal ligament fibroblasts,hPDLF)Ⅰ型膠原和纖連蛋白mRNA錶達的變化,為闡明力信號的細胞內轉導機製提供依據. 方法 對體外培養的hPDLF分彆施加不同動態張、壓應力(彊度為1000、2000、4000 μstrain,加力時間為0、0.5、1、4、8、12 h),定量聚閤酶鏈反應(PCR)檢測Ⅰ型膠原和纖連蛋白mRNA錶達. 結果 隨時間延長1000 μstrain張應力和壓應力均使Ⅰ型膠原和纖連蛋白mRNA錶達增彊,2000 μstrain和4000 μstrain張應力均使Ⅰ型膠原mRNA錶達先增彊1 h後減弱,而2000 μstrain和4000μstrain壓應力均使Ⅰ型膠原mRNA錶達持續減弱,2000 μstrain張應力和壓應力均使纖連蛋白mRNA錶達持續增彊,而4000 μstrain張應力和壓應力均使纖連蛋白mRNA錶達先增彊4 h後減弱. 結論 本項實驗提供的應力範圍內動態張應力和壓應力刺激可改變hPDLF Ⅰ型膠原和纖連蛋白mRNA的錶達.
목적 탐토궤계응력하인아주막성섬유세포(human periodontal ligament fibroblasts,hPDLF)Ⅰ형효원화섬련단백mRNA표체적변화,위천명력신호적세포내전도궤제제공의거. 방법 대체외배양적hPDLF분별시가불동동태장、압응력(강도위1000、2000、4000 μstrain,가력시간위0、0.5、1、4、8、12 h),정량취합매련반응(PCR)검측Ⅰ형효원화섬련단백mRNA표체. 결과 수시간연장1000 μstrain장응력화압응력균사Ⅰ형효원화섬련단백mRNA표체증강,2000 μstrain화4000 μstrain장응력균사Ⅰ형효원mRNA표체선증강1 h후감약,이2000 μstrain화4000μstrain압응력균사Ⅰ형효원mRNA표체지속감약,2000 μstrain장응력화압응력균사섬련단백mRNA표체지속증강,이4000 μstrain장응력화압응력균사섬련단백mRNA표체선증강4 h후감약. 결론 본항실험제공적응력범위내동태장응력화압응력자격가개변hPDLF Ⅰ형효원화섬련단백mRNA적표체.
Objective To investigate the effect of different dynamic tensional and compressive stress on the mRNA expression of collagen type Ⅰ and fibronectin in cultured human periodontal ligament fibroblasts (hPDLF), and explore the regularity of functional change in hPDLF. Methods A new cyclic strain loading apparatus was used for mechanically loading, Cells cultured in vitro were loaded with three levels (1000 μstrain, 2000 μstrain, 4000 μstrain) of tensional and compressive forces and collected at different time (0 h,0. 5 h,1 h,4 h,8 h,12 h) course after strain loading. The quantity of collagen type Ⅰ and fibronectin mRNA was analyzed by means of quantitative real-time PCR with special primers of up- and down-regulated genes. Data were analyzed using SPSS version 10. 0 software. Results Different magnitude and different kinds of mechanical forces as well as the force application time significantly changed the expression of collagen type Ⅰ and fibronectin mRNA in hPDLF. Conclusions Dynamic mechanical forces could regulate the expression of collagen type Ⅰ and fibronectin mRNA in hPDLF. Collagen type Ⅰ and fibronectin participated in the mechanical signal transduction in human periodontal ligament fibmblasts.
