CAJ | 학술논문

目的观察机械创伤大鼠对心肌缺血/再灌注损伤的敏感性.方法用Noble -Collip创伤仪制备机械创伤模型,采用完全随机分组方法将Wistar大鼠分为假创伤组、创伤组、假创伤+假手术组、假创伤+缺血/再灌组和创伤+缺血/再灌组,于创伤后1周行缺血/再灌注.采用BL - 410生物信号记录分析系统记录大鼠左心室收缩压(left ventricular systolic pressure,LVSP)、左心室压力上升和下降最大速率(±dp/dtmax)等心功能数据；采用双抗体夹心ABC -ELISA法检测大鼠血清肌酸激酶同工酶MB( CK - MB)、心肌肌钙蛋白(cTnI)水平；缺血/再灌注后心脏通过伊文思蓝染色、2,3,5 -氯化三苯基四氮唑(TTC)复染后,用Image - Pro Plus 6.0图像分析软件进行心肌梗死面积测定.结果创伤+缺血/再灌注组在体心功能明显低于假创伤+缺血/再灌注组(P＜0.01)；创伤+缺血再灌注组血清CK - MB和cTnI水平显著高于假创伤+缺血/再灌注组[ CK - MB:(4 960±588) ng/ml:(2 925±426) ng/ml,P＜0.01；cTnI:( 18.10 ±3.06) ng/ml:(6.67±1.57) ng/ml,P＜0.01]；创伤+缺血/再灌注组心肌梗死面积明显大于假创伤+缺血/再灌注组[(36.70±7.42)％:(22.27±4.54)％,P＜0.01].结论机械创伤使大鼠心肌对缺血/再灌注损伤的敏感性增加.
목적 관찰궤계창상대서대심기결혈/재관주손상적민감성.방법 용Noble -Collip창상의제비궤계창상모형,채용완전수궤분조방법장Wistar대서분위가창상조、창상조、가창상+가수술조、가창상+결혈/재관조화창상+결혈/재관조,우창상후1주행결혈/재관주.채용BL - 410생물신호기록분석계통기록대서좌심실수축압(left ventricular systolic pressure,LVSP)、좌심실압력상승화하강최대속솔(±dp/dtmax)등심공능수거；채용쌍항체협심ABC -ELISA법검측대서혈청기산격매동공매MB( CK - MB)、심기기개단백(cTnI)수평；결혈/재관주후심장통과이문사람염색、2,3,5 -록화삼분기사담서(TTC)복염후,용Image - Pro Plus 6.0도상분석연건진행심기경사면적측정.결과 창상+결혈/재관주조재체심공능명현저우가창상+결혈/재관주조(P＜0.01)；창상+결혈재관주조혈청CK - MB화cTnI수평현저고우가창상+결혈/재관주조[ CK - MB:(4 960±588) ng/ml:(2 925±426) ng/ml,P＜0.01；cTnI:( 18.10 ±3.06) ng/ml:(6.67±1.57) ng/ml,P＜0.01]；창상+결혈/재관주조심기경사면적명현대우가창상+결혈/재관주조[(36.70±7.42)％:(22.27±4.54)％,P＜0.01].결론궤계창상사대서심기대결혈/재관주손상적민감성증가.
Objective To observe the sensitivity of myocardium to ischemia/reperfusion (I/R) injury in mechanical trauma rats.Methods The mechanical trauma was established by Noble-Collip drum in the rats that were completely randomized into five groups:sham trauma group,trauma group,sham trauma and sham surgery group,sham trauma and I/R group,trauma and I/R group.The rats were subjected to 30 minutes of ischemia and one hour of reperfusion one week after trauma.The left ventricular systolic pressure (LVSP) and the left ventricular maximum rate of pressure rise and fall ( ± dp/dtmax ) were recorded with BL-410 biological signal recording and analysis system.The levels of serum creatine kinase isoenzyme MB (CK-MB) and cardiac troponinⅠ (cTnI) were detected by double antibody sandwich ABC-ELISA technique.At the end of reperfusion,the heart was excised and stained with Evan' s blue dye and triphenyltetrazolium chloride (TTC) to measure the infarct region with Image-Pro Plus 6.0 image analysis software.Results Compared with the sham traumatic I/R group,the cardiac function in vivo was significantly decreased in the traumatic I/R group ( P ＜ 0.01 ).While the serum CK-MB [ (4 960 ± 588 ) ng/ml:(2 925 ± 426) ng/ml,P ＜ 0.01 ],cTnI [ ( 18.10 ± 3.06 ) ng/ml:( 6.67 ±1.57 ) ng/ml,P ＜ 0.01 ] levels and myocardial infarct size [ ( 36.70 ± 7.42 ) ％:( 22.27 ± 4.54 ) ％,P＜0.01] were obviously higher in the traumatic I/R group compared with the sham traumatic I/R group.Conclusion Mechanical trauma increases the sensitivity of myocardium to I/R injury in rats.