中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
5期
404-406
,共3页
潘妹霞%YEUNG Victor%韩宏裕%耿庆山%许若军
潘妹霞%YEUNG Victor%韓宏裕%耿慶山%許若軍
반매하%YEUNG Victor%한굉유%경경산%허약군
染料木素%大豆黄素%海马%增殖%脑源性神经营养因子
染料木素%大豆黃素%海馬%增殖%腦源性神經營養因子
염료목소%대두황소%해마%증식%뇌원성신경영양인자
Genistein%Daidzein%Hippocampus%Brain-derived neurotrophic factor%Proliferation
目的 探讨染料木素和大豆黄素对海马神经细胞的活性和增殖作用及其可能机制.方法 H19-7/IGF-IR神经细胞在无酚红无血清DMEM培养液中培养72h后,分别加入一定浓度的染料木素、大豆黄素和雌二醇,用MTT和BrdU法分别检测细胞的活性和增殖,流式细胞术检测神经细胞周期,ELISA和RT-PCR法检测脑源性神经营养因子(BDNF)的表达.结果 处理细胞72h后,与对照组相比,20nM、200 Nm雌激素组H19-7细胞增殖分别提高了33%、36%;20 Nm、200 Nm染料木素组H19-7细胞增殖分别提高了15%、13%;200nM大豆黄素组H19-7细胞增殖分别提高了11%,差异有显著性(P＜0.05).200nM染料木素和大豆黄素的S期细胞比例[分别为(17.64±0.43)%,(19.48±1.01)%]显著高于对照组(14.21 ± 1.75)%,差异具有显著性(P＜0.05).染料木素和大豆黄素显著增加海马神经细胞成熟型BDNF水平及其Mrna的表达(P＜0.05).酪氨酸激酶受体阻断剂K252a能阻断染料木素和大豆黄素的促海马神经细胞增殖作用.结论 染料木素和大豆黄素改善海马神经细胞的增殖和活性能力,其作用与促进海马细胞BDNF的表达有关.
目的 探討染料木素和大豆黃素對海馬神經細胞的活性和增殖作用及其可能機製.方法 H19-7/IGF-IR神經細胞在無酚紅無血清DMEM培養液中培養72h後,分彆加入一定濃度的染料木素、大豆黃素和雌二醇,用MTT和BrdU法分彆檢測細胞的活性和增殖,流式細胞術檢測神經細胞週期,ELISA和RT-PCR法檢測腦源性神經營養因子(BDNF)的錶達.結果 處理細胞72h後,與對照組相比,20nM、200 Nm雌激素組H19-7細胞增殖分彆提高瞭33%、36%;20 Nm、200 Nm染料木素組H19-7細胞增殖分彆提高瞭15%、13%;200nM大豆黃素組H19-7細胞增殖分彆提高瞭11%,差異有顯著性(P＜0.05).200nM染料木素和大豆黃素的S期細胞比例[分彆為(17.64±0.43)%,(19.48±1.01)%]顯著高于對照組(14.21 ± 1.75)%,差異具有顯著性(P＜0.05).染料木素和大豆黃素顯著增加海馬神經細胞成熟型BDNF水平及其Mrna的錶達(P＜0.05).酪氨痠激酶受體阻斷劑K252a能阻斷染料木素和大豆黃素的促海馬神經細胞增殖作用.結論 染料木素和大豆黃素改善海馬神經細胞的增殖和活性能力,其作用與促進海馬細胞BDNF的錶達有關.
목적 탐토염료목소화대두황소대해마신경세포적활성화증식작용급기가능궤제.방법 H19-7/IGF-IR신경세포재무분홍무혈청DMEM배양액중배양72h후,분별가입일정농도적염료목소、대두황소화자이순,용MTT화BrdU법분별검측세포적활성화증식,류식세포술검측신경세포주기,ELISA화RT-PCR법검측뇌원성신경영양인자(BDNF)적표체.결과 처리세포72h후,여대조조상비,20nM、200 Nm자격소조H19-7세포증식분별제고료33%、36%;20 Nm、200 Nm염료목소조H19-7세포증식분별제고료15%、13%;200nM대두황소조H19-7세포증식분별제고료11%,차이유현저성(P＜0.05).200nM염료목소화대두황소적S기세포비례[분별위(17.64±0.43)%,(19.48±1.01)%]현저고우대조조(14.21 ± 1.75)%,차이구유현저성(P＜0.05).염료목소화대두황소현저증가해마신경세포성숙형BDNF수평급기Mrna적표체(P＜0.05).락안산격매수체조단제K252a능조단염료목소화대두황소적촉해마신경세포증식작용.결론 염료목소화대두황소개선해마신경세포적증식화활성능력,기작용여촉진해마세포BDNF적표체유관.
Objective To determine the effects of genistein and daidzein on the proliferation and survival of the hippocampal neural cells and underlying mechanism. Methods H19-7/IGF-IR neural cell line was cultured in phenol red free DMEM absented of serum for 72h. Genistein, daidzein or 17β-estradiol was added to the culture at various concentrations. Their proliferation and protective effects on the neuronal cells were determined by BrdU and MTT assay respectively. The effect of phytoestrogens on cell cycle regulation was determined using flow cytometry. The effects of the soy isoflavones on brain-derived neurotrophic factor (BDNF) expression were determined by ELISA and RT-PCR respectively. Results It was observed that, with 72h of treatment, 20nM and 200 nM 17B-estradiol significantly promoted the neuronal cell proliferation at 33% and 36% ;20nM and 200nM genistein significantly promoted the neuronal cell proliferation at 15% and 13% ; 200nM daidzein significantly promoted the neuronal cell proliferation at 11% compared to the control (P＜0.05). Genistein and daidzein induced an significantly increase in the S phase arrest at (17.64 ± 0.43) % and (19.48 ± 1.01) % compared to the control (P ＜ 0. 05). Moreover, genistein and daidzein significantly increased the expression of mature BDNF and BDNF mRNA level (P＜0.05). The effect of genistein and daidzein on hippocampal neuronal cell proliferation was blocked by K252a, selective inhibitor of tyrosine kinase receptors. Conclusion Genistein and daidzein improved hippocampal neuronal cell proliferation and viability in vitro. The effects might be mediated by increasing in BDNF expression.
