CAJ | 학술논문

目的探讨COX-2抑制剂NS398对鳞状细胞癌细胞Tca8113生长及凋亡的影响.方法细胞培养加入NS398作用后,采用噻唑蓝(MTT)法观察NS398对Tca8113细胞增殖的影响,流式细胞仪及透射电镜研究NS398对Tea8113细胞周期和凋亡的作用.结果 MTT比色法显示NS398能抑制Tca8113细胞的生长,呈浓度和时间依赖性.流式细胞仪检测结果显示NS398干预细胞出现典型的亚二倍体"凋亡峰";G0/G1期细胞比例升高,S期和G2/M期比例下降.电镜下见典型的凋亡形态学改变.结论 COX-2抑制剂NS398在体外可通过诱导凋亡有效抑制Tca8113细胞的生长.
목적 탐토COX-2억제제NS398대린상세포암세포Tca8113생장급조망적영향.방법 세포배양가입NS398작용후,채용새서람(MTT)법관찰NS398대Tca8113세포증식적영향,류식세포의급투사전경연구NS398대Tea8113세포주기화조망적작용.결과 MTT비색법현시NS398능억제Tca8113세포적생장,정농도화시간의뢰성.류식세포의검측결과현시NS398간예세포출현전형적아이배체"조망봉";G0/G1기세포비례승고,S기화G2/M기비례하강.전경하견전형적조망형태학개변.결론 COX-2억제제NS398재체외가통과유도조망유효억제Tca8113세포적생장.
Objective To study the effect of NS398, an inhibitor of cyclooxygenase 2 (COX2), on the growth and apoptosis of human squamous cell carcinoma cell line Tca8113. Methods Cultured Tca8113 cells were incubated with NS398 (0, 6.25, 12.5, 25, 50, 100 μmol/L) for 24, 48 and 72 hours, respectively. Thereafter, MTT method, flow cytometry and transmission electron microscopy were applied to detect the proliferation, cell cycle and apoptosis of Tca8113 cells, respectively. Results The proliferation of Tca8113 cells was inhibited by NS398 in a dose- and time-dependent manner (both P<0.05). FCM analysis showed the appearance of a typical hypodiploid apoptotic (Sub-G1) peak, an increase in the percentage of cells at G0/G1 phase and a decrease in that at S and G2/M phases in NS398 ( 100 μmol/L) -treated Tca8113 cells. Moreover, the cell proliferation index was significantly downregulated by NS398 of 100 μmol/L from 41.03 to 24.33 (P<0.05). Under an electron microscope, morphological changes characteristic of apoptosis were observed in NS398-treated Tca8113 cells. Conclusion NS398, an inhibitor of COX2, could effectively inhibit the growth of Tca8113 cells in vitro by induction of apoptosis.