中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2012年
5期
469-472
,共4页
郭友逢%张萌%李冰%江晓珍%李智
郭友逢%張萌%李冰%江曉珍%李智
곽우봉%장맹%리빙%강효진%리지
神经胶质瘤%肝细胞生长因子%血管形成
神經膠質瘤%肝細胞生長因子%血管形成
신경효질류%간세포생장인자%혈관형성
Glioma%Hepatocyte growth factor%Neovascularization
目的 探讨肝细胞生长因子(HGF)在人胶质瘤新生血管形成中的作用及可能机制.方法 中山大学附属第一医院病理科白2002年1月至2008年6月共确诊胶质瘤患者72例,其中Ⅰ级19例,Ⅱ级18例,Ⅲ级20例,Ⅳ级15例,免疫组化染色检测胶质瘤组织HGF、CD34的表达.体外常规培养胶质瘤细胞株U87MG,将荧光标记的HGF si-RNA(si-HGF)特异性干扰序列转染至细胞内为转染si-HGF组,同时设正常对照组和阴性转染组作为对照；Western blotting检测转染后细胞HGF、血管内皮生长因子(VEGF)的表达；将3组细胞悬液接种于鸡胚尿囊膜(CAM),5d后观察各组移植瘤周围血管形成情况. 结果 低级别胶质瘤(Ⅰ级、Ⅱ级)HGF阳性率低于高级别胶质瘤(Ⅲ级、Ⅳ级),HGF阳性胶质瘤中微血管密度(MVD)高于HGF阴性胶质瘤,差异均有统计学意义(P＜0.05).Western boltting检测结果显示,与正常对照组和阴性转染组比较,转染si-HGF组细胞HGF的表达下降约76％,VEGF的表达下降约53％；移植瘤实验显示转染si-HGF组新生血管数、血管面积及血管生成面积比均低于正常对照组和阴性转染组组,差异有统计学意义(P＜0.05). 结论 HGF与胶质瘤新生血管的形成关系密切,提示其有可能成为今后抗肿瘤血管生成的一个靶点.
目的 探討肝細胞生長因子(HGF)在人膠質瘤新生血管形成中的作用及可能機製.方法 中山大學附屬第一醫院病理科白2002年1月至2008年6月共確診膠質瘤患者72例,其中Ⅰ級19例,Ⅱ級18例,Ⅲ級20例,Ⅳ級15例,免疫組化染色檢測膠質瘤組織HGF、CD34的錶達.體外常規培養膠質瘤細胞株U87MG,將熒光標記的HGF si-RNA(si-HGF)特異性榦擾序列轉染至細胞內為轉染si-HGF組,同時設正常對照組和陰性轉染組作為對照；Western blotting檢測轉染後細胞HGF、血管內皮生長因子(VEGF)的錶達；將3組細胞懸液接種于鷄胚尿囊膜(CAM),5d後觀察各組移植瘤週圍血管形成情況. 結果 低級彆膠質瘤(Ⅰ級、Ⅱ級)HGF暘性率低于高級彆膠質瘤(Ⅲ級、Ⅳ級),HGF暘性膠質瘤中微血管密度(MVD)高于HGF陰性膠質瘤,差異均有統計學意義(P＜0.05).Western boltting檢測結果顯示,與正常對照組和陰性轉染組比較,轉染si-HGF組細胞HGF的錶達下降約76％,VEGF的錶達下降約53％；移植瘤實驗顯示轉染si-HGF組新生血管數、血管麵積及血管生成麵積比均低于正常對照組和陰性轉染組組,差異有統計學意義(P＜0.05). 結論 HGF與膠質瘤新生血管的形成關繫密切,提示其有可能成為今後抗腫瘤血管生成的一箇靶點.
목적 탐토간세포생장인자(HGF)재인효질류신생혈관형성중적작용급가능궤제.방법 중산대학부속제일의원병이과백2002년1월지2008년6월공학진효질류환자72례,기중Ⅰ급19례,Ⅱ급18례,Ⅲ급20례,Ⅳ급15례,면역조화염색검측효질류조직HGF、CD34적표체.체외상규배양효질류세포주U87MG,장형광표기적HGF si-RNA(si-HGF)특이성간우서렬전염지세포내위전염si-HGF조,동시설정상대조조화음성전염조작위대조；Western blotting검측전염후세포HGF、혈관내피생장인자(VEGF)적표체；장3조세포현액접충우계배뇨낭막(CAM),5d후관찰각조이식류주위혈관형성정황. 결과 저급별효질류(Ⅰ급、Ⅱ급)HGF양성솔저우고급별효질류(Ⅲ급、Ⅳ급),HGF양성효질류중미혈관밀도(MVD)고우HGF음성효질류,차이균유통계학의의(P＜0.05).Western boltting검측결과현시,여정상대조조화음성전염조비교,전염si-HGF조세포HGF적표체하강약76％,VEGF적표체하강약53％；이식류실험현시전염si-HGF조신생혈관수、혈관면적급혈관생성면적비균저우정상대조조화음성전염조조,차이유통계학의의(P＜0.05). 결론 HGF여효질류신생혈관적형성관계밀절,제시기유가능성위금후항종류혈관생성적일개파점.
Objective To investigate the effect ofhepatoeyte growth factor (HGF) on the neovascularization of human glioma and its mechanism. Methods Seventy-two patients with gliomas,admitted to our hospital from January 2002 to June 2008,including 19 with grade Ⅰ,18 with grade Ⅱ,20 with grade Ⅲ and 15 with grade Ⅳ,were chosen in our study.HGF and CD34 expressions in the paraffin section of gliomas in these 72 patients were detected with immunohistochemistry.Fluorescently labeled HGF-siRNA was transfected into U87MG glioma cells with TurboFect siRNA Transfection Reagent as si-HGF transfection group,and normal control group and negative transfection group were employed as controls; the HGF and vascular endothelial growth factor (VEGF) expressions in these U87MG cells were detected by Western blotting; cells fiom the 3 groups were inoculated to chorioallantoic membrane (CAM), and the angiogenesis ability of these U87MG cells were determined 5 d later. Results HGF-positive rate in patients with low-grade gliomas (grade Ⅰ and Ⅱ) was significantly lower than that in patients with high-grade glioma (grade Ⅲ and Ⅳ,P＜0.05); micro-vascular density (MVD) in gliomas with positive HGF was obviously higher than that in gliomas with negative HGF (P＜0.05).As compared with those in cells of the normal control group and negative transfection group, the HGF and VEGF expressions in cells of the si-HGF transfection group were descended by 76％ and 53％,respectively.The number and area of new vessels and the neovascularization ratio in cells of the si-HGF transfection group were signficantly decreased as compared with those in cells of the normal control group and negative transfection group (P＜0.05). Conclusion Close relation exists between HGF and neovascularization of gliomas,which indicates that HGF might become a new target for future antiangiogenesis treatment.
